Project description:HepG2 intact Hi-C For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:We developed a heat-inducible hepatic cell line (hi-Hep) in which BRCA1-associated protein-1 (BAP1) was introduced under the control of a TRE/PCMVmin promoter, in addition to the existing eight liver-enriched transcription factor (LETF) genes (HNF-1α, HNF-1β, HNF-3β, HNF-4α, HNF-6, C/EBP-α, C/EBP-β and C/EBP-γ). The synthetic heat-inducible promoter system utilizes a tetracycline-responsive transactivator (tTA) with a transcriptional positive-feedback loop and EGFP reporter, enabling stringent induction of transgenes upon transient heat treatment (43 °C, 30 min). Following induction, hi-Hep cells exhibit enhanced hepatic functions including ammonia detoxification, albumin secretion, and cytochrome P450 activity compared to parental HepG2 and HepG2/8F_HS cells. Transcriptome profiling was performed using Agilent SurePrint G3 Human GE 8×60K v3 arrays to compare hi-Hep cells with or without heat treatment, in monolayer and spheroid culture formats, under both serum-containing and optimized serum-free conditions, alongside parental HepG2/8F_HS controls.
Project description:HiC experiment done on HepG2 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Investigation of whole genome gene expression level changes in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Whole genome gene expression level changes have been compared in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Roche NimbleGen micro-array analysis was employed to assess global genome expression in HepG2 in regular culture, HepG2-slug in regular culture and HepG2-slug on Matrigel. The results demonstrated that the up-regulated genes and the down-regulated genes increased significantly when HepG2-slug cells with VM forming ablity were cultured on Matrigel and formed VM.
Project description:HepG2 cells were treated with 50 uM fangchinoline for 24 h. Proteomic profile of fangchinoline-treated HepG2 cells was compared with untreated cells.
Project description:Compare microRNAs expression when HepG2 cells stably expressing HBx protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase Compare microRNAs expression when HepG2 cells stably expressing URG11 protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase microRNA expression in Cells expressing HBx vs. Cell without HBx microRNA expression in Cells expressing URG11 vs. Cell without URG11
Project description:Two biological replicates Hi-C and HPV16-specific Region Capture Hi-C libraries were prepared for each of the W12 cell lines. Capture Hi-C was performed using HPV16-specific RNA baits. Hi-C libraries alone were prepared from normal cervix tissue (Ncx).