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Sorghum bicolor cultivar:RTx430

ABSTRACT: Sorghum bicolor RTx430 Standard Draft genome sequencing

PROVIDER: PRJNA460932 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR10566500_subreads.fastq.gz	Fastqsanger.gz
	SRR10566501_subreads.fastq.gz	Fastqsanger.gz
	SRR10566502_subreads.fastq.gz	Fastqsanger.gz
	SRR10566503_subreads.fastq.gz	Fastqsanger.gz
	SRR10566504_subreads.fastq.gz	Fastqsanger.gz

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Sorghum bicolor cultivar:RTx430

Project description:Sorghum bicolor cultivar:RTx430 Genome sequencing

| PRJNA337384 | ENA

Exploration of fine root gene expression relating to root pressure magnitude in Sorghum bicolor

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Novel drought adaptation strategies identified by comparing organelle- and cytoplasmic-enriched protein profiles of two Sorghum bicolor genotypes (RTx430 and BTx642) with differing pre-flowering drought tolerance

Project description:Proteomics of organelle and cytoplasmic enriched samples from Sorghum bicolor RTx430 (pre-flowering drought tolerant) and RTx642 (pre-flowering drought sensitive) before and during drought. 3 biological replicates, except BTx642 drought samples, which had 2 biological replicates due to limited sample. Identified multiple protein-level responses in both genotypes, demonstrated that these responses are consistent with the literature, as well as identified multiple RTx430-specific changes that suggest novel drought-tolerance strategies unique to RTx430. Sorghum leaves (both genotypes, +/- drought) were separated into cytosolic and organelle fractions, then digested with trypsin, and analyzed by LC-MS/MS. Data was searched using MaxQuant's Andromeda and a UniProt database. Data was then evaluated for missingness, normalized, and missing values imputed using Bioconductor R package DEP (Zhang et al. 2018).

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