Project description:Aim of this study was to compare proteomic profiles of the WT and the pMD8 mutant strains of Streptomyces albus. pMD8 is a mutant encoding a phosphoablative form of the CslA gene (T17A). Study is a part of a bigger project on the roles of PknB and CslA proteins in cell wall morphogenesis of Streptomyces. Another dataset related to this study is the PXD053764 phosphoproteomic analysis of the PknB deletion mutant strain vs the WT strain.This work was supported by Narodowe Centrum Nauki, 2020/39/D/NZ1/00303.The purchase of M-Class Acquity-Synapt XS LC–MS system was financially supported by the “Excellence Initiative – Research University” program for the University of Wroclaw, 2020–2025.
Project description:To evaluate the DNA binding of FscRI in Streptomyces albus S4 in vivo ChIP-Seq experiments were carried out on dusing anti-FLAG antibodies against 3xFLAG-FscRI protein using two biological replicates. The wild type strain was used as a control in experiments using the anti-FLAG antibodies as well as a DNA only control.
Project description:To evaluate the DNA binding of AntA in Streptomyces albus S4, in vivo ChIP-Seq experiments were performed 3xFLAG-AntA and anti-FLAG antibodies using two biological replicates. The wild type strain was used as a control in experiments using the anti-FLAG antibodies as well as a DNA only control (which were published previously, E-MTAB-5122).
Project description:Based on the genome sequencing and gene annotation, we analyzed the alginate lyases and other genes related to alginate metabolism of strain Vibrio sp. C42, with the highest alginate-degrading activity. Combined with proteome measurement, we proposed its alginate metabolic pathways.
