Project description:This experiment aimed at characterising the signalling pathways downstream SUCNR1 activation in murine Neural Stem Cells (NSCs). To this end, we profiled by microarray the gene expression changes induced by succinate stimulation in both wild type NSCs and GPR91-deficient NSCs.
Project description:To explore the mechanism and downstream pathways of PPT1 in hepatocellular carcinoma (HCC). We performed microarray assay to identify the downstream genes of PPT1. As a result, we obtained 476 (291 upregulation and 185 downregulation) differentially expressed genes significantly affected by PPT1 knockdown. Function enrichment analysis showed that these genes were enriched in “cell metabolism” and “signal transduction” pathways which are possibly related with lysosomal function. Real-time quantitative RT-PCR was performed to verify the microarray results. In conclusion, the expression profile of many genes were significantly affected by PPT1 knockdown, which helps to further explore the mechanism of the effects of PPT1 in HCC.
Project description:Within the overall project, we performed a set of microarray and chromatin-immunoprecipitation (ChIP)-chip experiments using siRNA against the (pro)renin receptor ((P)RR), stable overexpression of PLZF, the PLZF translocation inhibitor genistein and the specific V-ATPase inhibitor bafilomycin to dissect transcriptional pathways downstream of the (P)RR.
Project description:Within the overall project, we performed a set of microarray and chromatin-immunoprecipitation (ChIP)-chip experiments using siRNA against the (pro)renin receptor ((P)RR), stable overexpression of PLZF, the PLZF translocation inhibitor genistein and the specific V-ATPase inhibitor bafilomycin to dissect transcriptional pathways downstream of the (P)RR.