Synthetic construct
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA474936 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7275230.fastq.gz | Fastqsanger.gz | |||
| SRR7275231.fastq.gz | Fastqsanger.gz | |||
| SRR7275232.fastq.gz | Fastqsanger.gz | |||
| SRR7275233.fastq.gz | Fastqsanger.gz | |||
| SRR7275234.fastq.gz | Fastqsanger.gz |
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Project description:The goal of this CRISPR-based screen (Latency HIV-CRISPR) is to identify HIV-1 latency factors by evaluating multiple pathways simultaneously. Here are Illumina sequencing data and counts files from a Latency HIV-CRISPR screen using a custom guide RNA library targeting human epigenome genes (HuEpi). The Latency HIV-CRISPR screens described here were performed in clonal ZAP knockout J-Lat 10.6 (PMID: 12682019) or J-Lat 5A8 (PMID: 24204950) cells lines as ZAP inhibition of the HIV-CRISPR vector has been previously described (PMID: 30520725). The screens were performed in the presence or absence of AZD5582, a SMAC mimetic and latency reversal agent, in order to identify factors that are dependent and independent of this transcriptional activator.
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