Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Non-lytic clearance of influenza B virus from infected cells

ABSTRACT: Non-lytic clearance of influenza B virus from infected cells

PROVIDER: PRJNA476862 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7357970.fastq.gz	Fastqsanger.gz
	SRR7357971.fastq.gz	Fastqsanger.gz
	SRR7357972.fastq.gz	Fastqsanger.gz
	SRR7357973.fastq.gz	Fastqsanger.gz
	SRR7357964.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Non-lytic clearance of influenza B virus from infected cells [RNA-seq]

Project description:Non-lytic clearance of influenza B virus from infected cells [RNA-seq]

| PRJNA476582 | ENA

Non-lytic clearance of influenza B virus from infected cells [scRNA-seq]

Project description:Non-lytic clearance of influenza B virus from infected cells [scRNA-seq]

| PRJNA476864 | ENA

Non-lytic clearance of influenza B virus from infected cells [scRNA-seq]

Project description:Influenza B virus (IBV) is an acute respiratory pathogen that induces phenotypic alterations to the lung epithelium, such as the denudation of the respiratory cilium, during and after IBV infection. It has been assumed that these epithelial changes are non-adaptive, and simply the result of cellular death following lytic virus infection. However, previous reports have shown that not all infected cells are killed after viral infection; some cells can completely clear viral RNA and protein and persist in the host long-term. In this study, we utilized a novel recombinant virus in combination with a Cre recombinase-responsive transgenic mouse model to demonstrate that IBV infection leads to the formation of a population of survivor ciliated cells in the proximal airways of infected mice. To ensure that we were studying ciliated cells with markers that were maintained during viral infection in vivo, we performed single-cell transcriptional profiling on infected and surviving ciliated cell populations. We digested mouse lungs and sorted cells using tdTomato as a marker of infection. We sorted cell populations from the lungs of animals mock infected with PBS (Mock) and during active infection (2 DPI). The resulting data revealed that the marker CD24 is consistently expressed on ciliated cells before and during viral infection.

2018-12-05 | GSE116031 | GEO

Non-lytic clearance of influenza B virus from infected cells [RNA-seq]

Project description:Influenza B virus (IBV) is an acute respiratory pathogen that induces phenotypic alterations to the lung epithelium, such as the denudation of the respiratory cilium, during and after IBV infection. It has been assumed that these epithelial changes are non-adaptive, and simply the result of cellular death following lytic virus infection. However, previous reports have shown that not all infected cells are killed after viral infection; some cells can completely clear viral RNA and protein and persist in the host long-term. In this study, we utilized a novel recombinant virus in combination with a Cre recombinase-responsive transgenic mouse model to demonstrate that IBV infection leads to the formation of a population of survivor ciliated cells in the proximal airways of infected mice. We then performed transcriptional profiling on infected and surviving ciliated cell populations to determine how surviving viral infection affected these cells. To specifically profile ciliated cells, we digested mouse lungs and sorted cells based on their expression of CD24 with tdTomato as a marker of infection. We sorted cell populations from the lungs of animals mock infected with PBS (Mock), during active infection (2 DPI), and matched ciliated cell populations at 14 days post-infection that had either experienced direct viral infection (14 DPI Survivor Cell) or those that had never been infected (14 DPI). The resulting data indicate that after surviving infection and clearing the virus, survivor ciliated cells undergo significant transcriptional reprogramming.

2019-02-04 | GSE115952 | GEO

Non-lytic clearance of influenza B virus from infected cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-05 | GSE116032 | GEO

Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function.

Project description:Influenza B virus (IBV) is an acute, respiratory RNA virus that has been assumed to induce the eventual death of all infected cells. We and others have shown however, that infection with apparently cytopathic viruses does not necessarily lead to cell death; some cells can intrinsically clear the virus and persist in the host long-term. To determine if any cells can survive direct IBV infection, we here generate a recombinant IBV capable of activating a host-cell reporter to permanently label all infected cells. Using this system, we demonstrate that IBV infection leads to the formation of a survivor cell population in the proximal airways that are ciliated-like, but transcriptionally and phenotypically distinct from both actively infected and bystander ciliated cells. We also show that survivor cells are critical to maintain respiratory barrier function. These results highlight a host response pathway that preserves the epithelium to limit the severity of IBV disease.

| S-EPMC6377627 | biostudies-literature

Heterogeneity in antiviral responses in the upper respiratory tract promotes differential non-lytic clearance of influenza viruses

Project description:Heterogeneity in antiviral responses in the upper respiratory tract promotes differential non-lytic clearance of influenza viruses

| PRJNA606054 | ENA

Influenza virus infected HUVEC

Project description:To delineate specific patterns of signaling networks activated by H5N1 we used a comparative systems biology approach analyzing gene expression in endothelial cells infected with three different human and avian influenza strains of high and low pathogenicity.

2009-12-31 | GSE13637 | GEO

Influenza virus A infected monocytes

Project description:Gene expression profiles 6 hours post-influenza A virus infection in human monocytes at multiplicities of infection of 10 versus uninfected monocytes total six samples - comparing uninfected and infected samples from three individual subjects

2012-04-03 | E-GEOD-35473 | biostudies-arrayexpress

Human lung tissue explants infected with influenza B virus

Project description:RNAseq analysis of human lung tissue explants infected with Influenza B virus Results: Differentially expressed genes after infection Project: Hulu_FluB_2022

2022-08-31 | GSE197143 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data