Project description:Submergence is a type of abiotic stress that can severely impact the survival of plants and the yield of crops. Plants not only sense the hypoxic conditions during submergence but also exposed to a range of low-light intensities, depending on the submergence depth and turbidity. However, how light availability affects submergence tolerance remains largely unknown. To clarify the functions of light receptor AtPHYA and transcription factor AtEIN3/EIL1 in the transcriptional response to submergence, we conducted RNA-seq analysis on the wild type Col-0, the atphyA-211 mutant, and the atein3eil1 double mutant. Following a 6-hour submergence stress treatment, we identified 7325 differentially expressed genes (DEGs) in the Col-0 seedlings. The expression of 3748 submergence-induced genes was notably reduced in the atein3eil1 double mutant, while it was significantly enhanced in the atphyA-211 mutant. This suggests that AtPHYA exerts a negative regulatory effect on the expression of genes induced by submergence. The absence of AtPHYA leads to an upregulation of these submergence-induced genes, confirming that AtPHYA negatively modulates their expression.
Project description:To investigate the deposition of HTR5 in Arabidopsis, we analysed genome-wide HTR5 density in the wild-type Col-0 by ChIP-seq. We then performed HTR5 occupancy analysis using data obtained from ChIP-seq of 3 different plants including HA-HTR5/Col-0 and Col-0. Col-0 acted as negative control.
Project description:Transcriptional profiling of 60h-old Arabidopsis whole seedlings comparing control Col-0 wild-type plants with pifQ mutant plants The expression profile of dark-grown pifQ mutant shows similar pattern of Rc-grown Col-0 wild-type Keywords: Genetic modification
Project description:Hypoxia exerts major effects on gene expression, which is reconfigured to meet the needs of the stressed plant. In the recent years the mechanisms of oxygen sensing and transcriptional regulation of a cluster of anaerobic genes was described. We utilized a hypomorphic, post-transcriptional gene silencing defective ARGONAUTE1 (AGO1) mutant, ago1-27, to evaluate the contribution of AGO1 to plant tolerance to submergence and its effects on gene expression.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus.