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Peripheral Blood Transcriptomic Analysis for Salmonella Infection in Pig

ABSTRACT: Peripheral Blood Transcriptomic Analysis for Salmonella Infection in Pig

PROVIDER: PRJNA484712 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7647440.fastq.gz	Fastqsanger.gz
	SRR7647431.fastq.gz	Fastqsanger.gz
	SRR7647441.fastq.gz	Fastqsanger.gz
	SRR7647432.fastq.gz	Fastqsanger.gz
	SRR7647442.fastq.gz	Fastqsanger.gz

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Peripheral Blood Transcriptomic Analysis for Salmonella Infection in Pig

Project description:Peripheral blood transcriptome is an important intermedia data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four weeks old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood gene expression profile before treatment (d0) and at 2 and 7 day post inoculation (dpi) using deep sequencing technology.

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Project description:Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of this gram-negative bacteria in such pigs is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonella shedding following experimental inoculation, we have initiated the first analysis of the whole blood transcriptional response induced by Salmonella. A population of pigs (n=40) was inoculated with ST and the peripheral blood and feces were collected between 2 and 20 days post-inoculation. Two groups of pigs with either low shedding (LS) or persistent shedding (PS) phenotypes were identified. The global transcriptional changes in response to ST inoculation were identified by Affymetrix Genechip?analysis of peripheral blood RNA at day 0 and day 2 post-inoculation.

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Peripheral Blood Transcriptomic and miRNA Transcriptomic Analyses for Salmonella Infection in Pig

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