Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of 5' UTR structures in Listeria monocytogenes

ABSTRACT: Comparison of 5' UTR structures in Listeria monocytogenes

PROVIDER: PRJNA485422 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7666040_1.fastq.gz	Fastqsanger.gz
	SRR7666040_2.fastq.gz	Fastqsanger.gz
	SRR7666041_1.fastq.gz	Fastqsanger.gz
	SRR7666041_2.fastq.gz	Fastqsanger.gz
	SRR7666042_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Comparison of 5' UTR structures in Listeria monocytogenes

Project description:To understand physiological processes in vivo, it is vital to disclose the conformation of biological molecules inside living cells and correlate their structure with their function. Here, we describe a method, FUSE (5’-UTR Structure Elucidation) that was used to characterize changes in the structures of 5’-UTRs at different conditions inside the human bacterial pathogen Listeria monocytogenes. Using FUSE, we discovered a novel RNA thermoswitch and calculated the ratio of two conformations that this thermoswitch assumes at different conditions. FUSE could also identify the site of base-pairing interaction between a small RNA and mRNA with a single-nucleotide resolution. Strikingly, FUSE discovered a functional mRNA-mRNA interaction, where the stability of mRNA encoding PrsA2 chaperone, was increased by the direct binding of an mRNA encoding its substrate, listeriolysin O, the main L. monocytogenes virulence factor. Owing to its focus on 5’-UTRs, FUSE can be applied to analyse these regulatory regions in any living organism.

2018-08-11 | GSE118387 | GEO

Listeria monocytogenes transcriptional response to bile

Project description:These studies were designed to examine the acute Listeria monocytogenes transcriptional response to mammalian (porcine) bile.

2011-04-12 | GSE28507 | GEO

CtsR Regulon in Listeria monocytogenes

Project description:The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator CtsR, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DctsR log phase cells were compared to both wt and ictsR-mcsA log phase cells grown with 0.5mM IPTG to identify CtsR-dependent genes.We identified 62 CtsR-dependent genes that showed significant expression ratios (adj. P < 0.05), with ≥ 1.5-fold differential expression either between ΔctsR and wt or between ΔctsR and ictsR-mcsA. Keywords: Listeria monocytogenes, CtsR regulon, log phase

2007-10-14 | GSE7514 | GEO

HrcA Regulon in Listeria monocytogenes

Project description:The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator HrcA, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DhrcA stationary phase cells were compared to wt to identify hrcA-dependent genes. We identified 61 HrcA-dependent genes that showed significant expression ratios (adj. P < 0.05), with ≥ 1.5-fold differential expression between ΔhrcA and wt. Combined with microarray analysis, Hidden Markov Model searches show HrcA directly repress at least 8 genes. Keywords: Listeria monocytogenes, HrcA regulon, stationary phase

2007-11-03 | GSE7517 | GEO

Listeria monocytogenes exposure to chlorine dioxide

Project description:Listeria monocytogenes strain 10403S has been studied extensively for stress response activity toward multiple stressors (acid, osmotic, cold, high temperature, etc.) as well as multiple stress regulons (SigB, CtsR, HrcA, etc.). Here we aimed to determine the transcriptional response of Listeria monocytogenes in early log phase towards the strong oxidative stress imposed by ClO2. The elucidation of such a response allows for further a more completel understanding of the mechanism of inactivation by sanitizers, specifically ClO2.

2014-03-07 | GSE48187 | GEO

Gene expression profile in peritoneal macrophage infected with Listeria monocytogenes

Project description:Several Toll-like receptors are activated by Listeria monocytogenes infection, resulting in the activation of MyD88 dependent signaling pathway. However, the negative role of MyD88 in gene expresson is unclear. To address this, we performed microarray analysis of mRNAs from WT or MyD88-/- peritoneal macrophages infected with Listeria monocytogenes.

2014-06-29 | GSE52115 | GEO

Intracellular transcription by multiple Listeria monocytogenes strains

Project description:These studies were designed to examine the transcription of Listeria monocytogenes strains 10403S and LO28 during intracellular replication in mammalian macrophages.

2011-12-02 | GSE34083 | GEO

Surface Proteins of Listeria monocytogenes

Project description:Listeria monocytogenes cells (strain LI0521) were digested with trypsin for the identification of surface proteins. The supernatant was filter-sterilized and subjected to identification by LC-MS/MS. Concurrently secreted or shed proteins were identified by isolating filter-sterilized supernatants following incubation of L. monocytogenes cells in buffer without trypsin. This was followed by trypsin digest of the sterilized supernatant and identification by LC-MS/MS.

2013-08-27 | PXD000035 | Pride

Listeria monocytogenes

Project description:Expression profile comparison among Listeria monocytogenes strains

| PRJNA92891 | ENA

Project description:Comparison of Listeria monocytogenes 5' UTR structures

| PRJNA484729 | ENA