Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

ABSTRACT: AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

PROVIDER: PRJNA486456 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7704750.fastq.gz	Fastqsanger.gz
	SRR7704751.fastq.gz	Fastqsanger.gz
	SRR7704752.fastq.gz	Fastqsanger.gz
	SRR7704745.fastq.gz	Fastqsanger.gz
	SRR7704746.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

Project description:We compared polyIC stimulated cells in the presence of either GFP or NS5 protein

2018-08-18 | GSE118686 | GEO

Leo1 occupancy in presence of dengue virus NS5

Project description:We use ChIP-seq on PAF1C member Leo1 to determine how PAF1C occupancy at interferon stimulated genes is impacted by dengue virus NS5 protein.

2018-12-03 | GSE110511 | GEO

JEV and ZIKV NS5 interactome

Project description:Among the flaviviral proteins, NS5 is the largest and most conserved. NS5 contains major enzymatic components of the viral replication complex. Disruption of the common key NS5-host protein-protein interactions critical for viral replication could aid in the development of broad-spectrum anti-flaviviral therapeutics. To this end, we investigated the JEV- and ZIKV-NS5 interactomes in human cells using GFP pull-downs with mass spectrometry analysis in a label-free fashion. A total of 138 cellular proteins interacting with NS5 from JEV, ZIKV, or both were identified as Protein classification analysis of identified cellular targets revealed the enrichment of RNA binding, processing and splicing including spliceosomal and spliceosome-associated proteins in both datasets. Comparison of our data with literature not only revealed several cellular NS5 interacting proteins shared among flaviviruses, but also identified proteins that have no known function in flavivirus biology such as RNA polymerase II-associated Paf1 complex, protein phosphatase 6, and s-adenosylmethionine synthetase. Our study generates the first landscape of the JEV and ZIKV NS5 interactome in human cells and identifies cellular proteins that are potentially targetable for broad-spectrum anti-flaviviral therapy.

2022-05-04 | PXD009523 | Pride

DHS NS5 Cells

Project description:DHS NS5 Cells

| PRJEB5275 | ENA

Leo1 occupancy in presence of dengue virus NS5

Project description:Leo1 occupancy in presence of dengue virus NS5

| PRJNA433884 | ENA

HDX-MS Studies of Dengue Virus Serotype 2 NS5 (DENV2 NS5) and Stem Loop A (SLA) Interactions

Project description:The project employs HDX-MS to study the conformational dynamics of DENV2 NS5 in its apo form and in complex with the viral promoter RNA, Stem Loop A (SLA). The conformational dynamics of the RdRp domain of DENV2 NS5 (NS5-RdRp) in its apo form and in complex with SLA was also studied.

2025-05-07 | PXD058297 | Pride

ChIP NS5 Ascl1-ERT2

Project description:ChIP NS5 Ascl1-ERT2

| PRJEB5781 | ENA

ChIP-seq NS5 RBPJ

Project description:ChIP-seq NS5 RBPJ

| PRJEB12778 | ENA

ChIP-seq NS5 Zeb1

Project description:ChIP-seq NS5 Zeb1

| PRJEB9360 | ENA

Differential gene expression in murine neural stem cells (NS5) and neural stem cell-derived astrocytes

Project description:Gene expression in NS5 murine neural stem cells was compared to that of astrocytes derived from NS5 cells via 3 days of treatment with foetal calf serum (FCS). Keywords: cell type comparison

2009-12-31 | GSE10721 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data