Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Extraction and Biomolecular Analysis of Dermal Interstitial Fluid Collected with Hollow Microneedles

PROVIDER: PRJNA489285 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7784470.fastq.gz	Fastqsanger.gz
	SRR7784480.fastq.gz	Fastqsanger.gz
	SRR7784490.fastq.gz	Fastqsanger.gz
	SRR7784500.fastq.gz	Fastqsanger.gz
	SRR7784471.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 33

Similar Datasets

Proteomic Characterization of Human Dermal Interstitial Fluid

Project description:Proteomic characterization of human dermal interstitial fluid compared to matching serum and plasma.

2019-05-21 | PXD012658 | Pride

A Microneedle Device for Rapid Dermal Interstitial Fluid Sampling

Project description:Dermal interstitial fluid (ISF) shows promise for novel skin diagnostics and as an alternative to invasive blood tests. This dataset includes ISF sampled via a microneedle device from two ex vivo human abdominal skin specimens and the forearms of ten healthy volunteers. Paired blood samples via finger prick were also collected from five volunteers. We studied the ISF proteome in relation to collection time and pressure ex vivo. We also created a reference ISF proteome from the healthy volunteers, compared it to the plasma proteome, and quantified errors from blood contamination.

2025-08-23 | PXD064496 | Pride

Time-dependent stability of plasma and liver interstitial fluid metabolites during sample handling in mice

Project description:<p>This study assesses the stability of metabolite levels in plasma and liver interstitial fluid (IF) from mice subjected to different handling times prior to extraction. Blood and tissues from female C57BL/6J mice were collected and either processed immediately or held on ice for 5, 10, or 30 minutes before analysis. Metabolite concentrations were quantified by LC/MS to determine how delays in processing influence metabolite integrity. The resulting data establish time-dependent changes in metabolite abundance during pre-analytical handling.</p>

2025-12-30 | MTBLS13164 | MetaboLights

Neonatal bone marrow interstitial fluid supports expansion and osteogenic ability of human bone marrow mesenchymal stromal cells

Project description:The bone marrow microenvironment is critical for the maintenance and functionality of stem/progenitor cells, which is essential for bone development and regeneration. However, the composition and potential use of bone marrow interstitial fluid have not been extensively explored. In this study, we report a role of neonatal bovine bone marrow interstitial fluid (NBIF) in enhancing bone regeneration capacity of human bone marrow mesenchymal stem cells (hBMSCs). Unlike adult bovine bone marrow interstitial fluid (ABIF), NBIF-fed hBMSCs exhibit enhanced self-renewal and osteogenic potential and bone marrow homing ability with transcriptome changes as against hBMSCs cultured in standard fetal bovine serum (FBS) supplemented medium. Mass spectrometry analysis reveals that multiple secreted factors associated with tissue repair and bone development are enriched in NBIF compared to FBS and ABIF. The combined use of NBIF-enriched Nerve Growth Factor (NGF), Lactoferrin (LTF) and High Mobility Group Protein B1 (HMGB1) with Insulin-Like Growth Factor 1(IGF1) for culturing hBMSCs in the presence of FBS can enhance osteogenic potential and bone marrow homing ability, mimicking NBIF’s effects. These findings highlight the role of interstitial fluid in the bone marrow microenvironment and its potential to optimize stem cell based therapies.

2025-12-30 | GSE290284 | GEO

LC-MS/MS analysis of tumour interstitial fluid from human breast cancers

Project description:Proteomics analysis of interstitial fluid from 35 human breast cancers.

2020-11-11 | PXD001686 | Pride

RNA-seq of mononuclear immune cells isolated from breast cancer wound fluid after IORT or surgery alone

Project description:This dataset comprises RNA-seq profiles from mononuclear immune cells purified from wound fluid of breast cancer patients who received intraoperative radiotherapy (IORT) or surgery only (control). Samples were collected on postoperative day 2 and processed for total RNA extraction and 3′-end mRNA sequencing. The study aims to characterize the transcriptional immune landscape in the early wound-healing phase following IORT.

2026-04-07 | GSE302811 | GEO

2a8d9e3c-3a68-4472-b4b7-40985237aca5 - samples

Project description:CTD-ILD BALF and blood scRNA-seq dataset consists of single-cell transcriptome data of bronchoalveolar lavage fluid (BALF) and blood derived from 30 connective tissue disease-associated interstitial lung disease (CTD-ILD) and 12 idiopathic interstitial pneumonia (IIP) patients.

| EGAD00001011334 | EGA

Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways

Project description:Osteocytes, positioned within boneâs interstitial space, are subject to fluid flow upon whole bone loading. Such fluid flow is widely theorized to be a mechanical signal transduced by osteocytes, initiating a poorly understood cascade of signaling events mediating bone metabolism. The objective of this study was to utilize high-throughput approaches to examine the time course of flow-induced changes in osteocyte gene transcript and protein levels. Microarray analysis demonstrated fluid flow regulation of genes consistent with known anabolic loading responses, including Ptgs2, NF-ÎºB inhibitors, MAP3 kinases, and Wnt/Î²-catenin pathway signaling molecules. However, two of the most highly up-regulated gene productsâCxcl1 and Cxcl2, confirmed by qPCRâhave not previously been reported to be responsive to fluid flow. Gene ontology analysis suggested a highly significant inflammatory and immune response, with cellular functions including trafficking, cell-to-cell signaling, and tissue development. Proteomic analysis of the same samples demonstrated greatest up-regulation of the ATP-producing enzyme NDK, calcium-binding Calcyclin, and G protein-coupled receptor kinase 6. An integrative pathway analysis merging fold changes in transcript and protein levels predicted signaling nodes not directly detected at the sampled time points, including STAT3 and c-Myc. These results extend our knowledge of the osteocytic response to fluid flow, most notably up-regulation of Cxcl1 and Cxcl2 as a possible paracrine agent for osteoblastic and osteoclastic recruitment. Osteocyte-like MLO-Y4 cells were subjected to 2 hours of 10 dyn/cm2 oscillating fluid flow in parallel-plate fluid flow chambers and harvested for analysis at 0, 2, 8, and 24 hours post-flow incubation. Parallel control samples from sham treated cells were also collected at each time point.

2013-07-01 | E-GEOD-42874 | biostudies-arrayexpress

Utilization of interstitial fluid extract (IFE) to model cancer in the context of old age in vitro.

Project description:Interstitial fluid is the biological fluid that occupies the spaces between cells within tissues, acting as a vital medium for the exchange of nutrients and waste products. It reflects not only the composition of specific microenvironment of an organ but is also reflective of the tumor milieu, including secreted factors and small molecules from both tumor and normal cells. As such, interstitial fluid captures a biologically relevant snapshot of the organ’s microenvironment, whether cancer is present or not. Here we introduce interstitial fluid extract (IFE) and present a protocol for its isolation from the lungs of metastasis-bearing mice, followed by ex vivo treatment of cultured cells and high-throughput transcriptomic analysis. This approach is highly flexible, supporting a variety of downstream applications and adaptable to different experimental formats such as mouse strain, cancer cell type, and organ of interest. By comparing the molecular profiles of IFE-treated cells with those of cells sorted directly from metastasis-bearing lungs, the protocol helps validate the biological relevance of the IFE model. This approach is particularly valuable for studying in vivo-like conditions where direct in vivo experiments are challenging or not feasible, such as in the context of old age, offering a practical alternative for investigating complex tumor microenvironments and cellular responses.

2025-12-09 | GSE302268 | GEO

Transcriptome analysis of cells in mouse hairy melanocytic nevi

Project description:RNA-seq data was obtained from different skin cell types at two time points: postnatal day P30, when WT dorsal hair follicles are in actively growing state (aka anagen) and P56, when WT hair follicles are in resting state (aka telogen). Cell types include: Bulge stem cells (B), dermal papilla fibroblasts (DP), secondary hair germ cells (HG), melanocytes (Melan), myeloid cells (Myel) and CD45-positive immune cells (I). All cell were FACS sorted and only live single cells were collected for RNA extraction.

2023-03-14 | GSE111999 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data