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ABSTRACT: Native AMF Diversity

PROVIDER: PRJNA490254 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7817725.fastq.gz	Fastqsanger.gz
	SRR7817726.fastq.gz	Fastqsanger.gz

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Wheat proteome in response to Plant Growth Promoting Bacteria (PGPB) and Arbuscular Mycorrhizal Fungi (AMF) treatment

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Project description:Purpose: To date, the biological activity of AMF has not been fully investigated. We set out to analyze how AMF regulates gene expression in HepG2 cells. Conclusions: we performed clustering analysis on the various expressed genes associated with autophagy, and the pathways confirmed in the KEGG and BP analysis

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Project description:Traditional surgery plus radiotherapy or chemotherapy, existing targeted therapies failed to significantly improve the survival rate of recurrent endometrial cancer, so suggesting that mechanism of recurrence and progression that modulates in endometrial cancer is clinically important. Here, we show that GPER(G protein-coupled estrogen receptor 1) was binded to AMF, and the complex were translocation form plasma to cytoplasmic. Mechanistic investigations elucidated that interaction of AMF with GPER triggers phosphoinositide-3-kinase (PI3K) signaling activating and accelerating the ability of endometrial cancer cells growth. Furthermore, we found that AMF may contribute to GPER-mediated endometrial cancer progression using animal experiments and human histological experiments which be consistent with the above conclusions. On the basis of these evidences including invivo and invitro, our findings suggest that AMF–GPER interaction might be novel key molecular targets for therapeutic management of patients with endometrial cancer, whose disease were progression and recurrence.

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Effect of plant growth-promoting bacteria (PGPB), arbuscular mycorrhizal fungi (AMF), and their consortia on wheat HD-3086 grain proteome at maturity stage (under field conditions; second year of field trial)

Project description:The current study is aimed at elucidating the proteomic responses in the seed tissue of durum wheat Triticum aestivum L. (cultivar HD-3086) triggered by native (CP4) and non-native (AHP3) PGPB (Bacillus spp.) alone and in combination with AM fungi (Glomus fasciculatum + Gigaspora sp.) under field conditions. Our results suggest that application of CP4 and AHP3 (in combination with AM fungi) promote differential abundance of multiple regulatory seed storage and other proteins over untreated control. Thus, combined application of tripartite (CP4+AHP3+AMF) microbial consortia could offer a more sustainable approach to enhance crop yield in wheat.

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