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Small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [smRNA-Seq]

ABSTRACT: small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [smRNA-Seq]

PROVIDER: PRJNA493072 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7900250.fastq.gz	Fastqsanger.gz
	SRR7900251.fastq.gz	Fastqsanger.gz
	SRR7900252.fastq.gz	Fastqsanger.gz
	SRR7900253.fastq.gz	Fastqsanger.gz
	SRR7900254.fastq.gz	Fastqsanger.gz

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small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [smRNA-Seq]

Project description:We report the role of sRNAs populations during the induction of callus tissues from VS-535 maize embryos displaying contrasting in vitro embryogenic potential; characterized through Next-generation sequencing (NGS). We conclude that the Embryogenic Response during Maize Somatic Embryogenesis induction is closely related to sRNAs regulation and depends on the developmental stage of the explant.

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small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis

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Epigenetic modifications and their relationships to smRNA and mRNA transcriptomes in maize

Project description:Genome-wide and organ-specific landscapes of epigenetic modifications and their relationships to mRNA and smRNA transcriptomes in maize We report an integrated genome-wide analysis of DNA methylation, histone modifications, smRNAs and mRNA transcriptional activity, using maize as a model. We surveyed the epigenomes of the maize inbred line B73 in shoot and root tissue by Illumina/Solexa 1G parallel sequencing after digesting genomic DNA with a methylation-sensitive restriction enzyme and after conducting chromatin immunoprecipitations (ChIP) using antibodies that target specific histone modifications (H3K4me3, H3K9ac, H3K27me3, H3K36me3, respectively). Additionally, we profiled RNA pools (micro RNA (miRNA), siRNA and mRNA) using the same sequencing strategy. Keywords: Epigenetics, mRNA transcription and small RNAs

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