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Host cell gene expression profile in vaccinia virus infected mouse RAW cells at 2, 16 hours post infection

ABSTRACT: Host cell gene expression profile in vaccinia virus infected mouse RAW cells at 2, 16 hours post infection

PROVIDER: PRJNA494080 | ENA |

REPOSITORIES: ENA

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Host cell gene expression profile in vaccinia virus infected mouse RAW cells at 2, 16 hours post infection

Project description:The poxviruses are a family of linear double-stranded DNA viruses about 130 to 230 kbp, that belong to the family Poxviridae. The poxviruses have an animal origin and have evolved to infect a wide host range. Variola virus (VARV), the causative agent of smallpox, is a poxvirus that infect only humans, but other poxviruses such monkey pox virus and cowpox virus have also across over from animals to infect humans. Therefor understanding the biology of poxviruses can help to devise antiviral strategies. In this study we used a system-based approach to examine the host responses to three different orthopoxviruses, CPXV, VACV and ECTV in the murine macrophage RAW 264.7 cell line.

2019-01-15 | GSE120614 | GEO

Host cell gene expression profile in cowpox virus infected mouse RAW cells at 2, 16 hours post infection

Project description:Host cell gene expression profile in cowpox virus infected mouse RAW cells at 2, 16 hours post infection

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Host cell gene expression profile in ectromelia virus infected mouse RAW cells at 2, 16 hours post infection

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Effect of LPS and LPS-binding protein treatment for 16 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 16 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 16 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

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Host cell gene expression profile in cowpox virus infected mouse RAW cells at 2, 16 hours post infection

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Host cell gene expression profile in ectromelia virus infected mouse RAW cells at 2, 16 hours post infection

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Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus infected cells reveals virus-specific regulation of immune response genes

Project description:Orthopoxviruses are large DNA viruses which can cause disease in numerous host species. Even though the eradication of variola virus - the causative agent of human smallpox – succeeded, with the end of vaccinations several other orthopoxviruses emerged as potential threat to human health. For instance, animal-borne monkeypox virus, cowpox virus and closely related vaccinia virus are all capable of establishing zoonotic infections in humans. The disease caused by each virus differs in terms of expression and severity, but we still know little about the reasons for these different phenotypes. They may be explained by the unique repertoire of host cell modulating factors encoded by each virus. In this study, we aimed at characterizing the specific modulation of the host cells gene expression profile by orthopoxvirus infection. In our study we analyzed changes in host cell gene expression of HeLa cells after infection with cowpox virus, monkeypox virus or vaccinia virus and compared these to each other and to the gene expression profile of non-infected cells using Agilent Whole Genome Microarray technology. We could identify major differences in viral modulation of host cell immune response genes, especially an induction of genes involved in leukocyte migration and Toll-like receptor signalling in cowpox and monkeypox virus infected cells. This was not observed following vaccinia virus infection. If these differences contribute to the different clinical manifestation of cowpox, monkeypox and vaccinia virus infections in certain host species remains to be elucidated.

2012-04-15 | GSE36854 | GEO

Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus infected cells reveals virus-specific regulation of immune response genes

Project description:Orthopoxviruses are large DNA viruses which can cause disease in numerous host species. Even though the eradication of variola virus - the causative agent of human smallpox M-bM-^@M-^S succeeded, with the end of vaccinations several other orthopoxviruses emerged as potential threat to human health. For instance, animal-borne monkeypox virus, cowpox virus and closely related vaccinia virus are all capable of establishing zoonotic infections in humans. The disease caused by each virus differs in terms of expression and severity, but we still know little about the reasons for these different phenotypes. They may be explained by the unique repertoire of host cell modulating factors encoded by each virus. In this study, we aimed at characterizing the specific modulation of the host cells gene expression profile by orthopoxvirus infection. In our study we analyzed changes in host cell gene expression of HeLa cells after infection with cowpox virus, monkeypox virus or vaccinia virus and compared these to each other and to the gene expression profile of non-infected cells using Agilent Whole Genome Microarray technology. We could identify major differences in viral modulation of host cell immune response genes, especially an induction of genes involved in leukocyte migration and Toll-like receptor signalling in cowpox and monkeypox virus infected cells. This was not observed following vaccinia virus infection. If these differences contribute to the different clinical manifestation of cowpox, monkeypox and vaccinia virus infections in certain host species remains to be elucidated. We analyzed the gene expression profile of HeLa cells wich were either mock-infected or infected with Vaccinia virus strain IHD-W, Cowpox virus strain Brighton Red or Monkeypox virus strain MSF#6 at a multiplicity of infection of 5. Experiments were performed in duplicate. At 6 h post infection total RNA was isolated from infected cells and used for microarray analysis.

2012-04-14 | E-GEOD-36854 | biostudies-arrayexpress

MCF7 cells treated with hypoxia for 0, 5, 10, 16 hours

Project description:To investigate the hypoxia-regulated gene, we treated MCF7 cells with hypoxia for 0, 5, 10, 16 hours.

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Fission yeast Cdc10-YFP interactome in MM-N 16 hours

Project description:Mass spectrometry analysis of proteins co-purifying with Cdc10, a component of the MBF transcriptional complex, was performed both in a wild-type and in a whi5∆ background. Cdc10 was tagged with YFP at its C-terminal end and expressed from its endogenous promoter at its chromosomal locus. Cdc10-YFP was immunoprecipitated from cells cultured in MM-N medium for 16 hours, and co-purifying proteins were analysed by mass spectrometry. An untagged wild-type strain was used as a control.

2025-12-20 | PXD057838 | Pride

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