Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

ABSTRACT: Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

PROVIDER: PRJNA494083 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7945410.fastq.gz	Fastqsanger.gz
	SRR7945420.fastq.gz	Fastqsanger.gz
	SRR7945430.fastq.gz	Fastqsanger.gz
	SRR7945440.fastq.gz	Fastqsanger.gz
	SRR7945450.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 47

Similar Datasets

Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

Project description:The goal of this study was to identify microRNAs that are modulated by cyclic stretch. miRNA-Seq was performed comparing samples from cultured E16.5 mouse cardiomyocytes exposed to either cyclic stretch of 16% at 1Hz for 24h or static conditions.

2020-01-22 | GSE120676 | GEO

Hypergravity Enhances Stretch Sensitivity in Rat Cardiomyocytes via Increased Expression and Activity of Stretch-Activated Channels

Project description:This study investigated how 14-day 4g hypergravity exposure affects mechanosensitive ion channels (MSCs) in rat ventricular cardiomyocytes. RNA-seq revealed selective MSC remodeling: TRPM7 (+41%), TRPC1 (+68%), and TRAAK (+239%) were upregulated, while TRPV2 (-62%), Piezo2 (-58%), and Kir6.1 (-76%) were downregulated. Structural analysis showed 44% increased membrane capacitance and sarcomere elongation, consistent with hypertrophic remodeling. Patch-clamp studies demonstrated markedly hypersensitive stretch-activated currents (I_SAC), with activation at minimal displacements (1-2 μm) and enhanced responses under 4-10 μm stretch (107-222% of controls). These coordinated molecular, structural, and functional adaptations suggest targeted transcriptional reprogramming that balances enhanced Ca²⁺ signaling with stabilized K⁺ conductance, demonstrating cardiac mechanotransduction plasticity under gravitational stress with implications for cardiovascular risks in altered gravity environments.

2025-12-02 | GSE306309 | GEO

Comparative analysis of time-dependent effects of dynamic stretch on gene expression in neonatal rat cardiomyocytes

Project description:The present study focuses on the identification of the gene expression profile of neonatal rat cardiomyocytes (NRVCMs) after dynamic mechanical stretch through microarrays of RNA isolated from cells stretched for 2, 6 or 24 h.

2016-12-29 | GSE92956 | GEO

Hypergravity Enhances Stretch Sensitivity in Rat Cardiomyocytes via Increased Expression and Activity of Stretch-Activated Channels

Project description:Hypergravity Enhances Stretch Sensitivity in Rat Cardiomyocytes via Increased Expression and Activity of Stretch-Activated Channels

| PRJNA1310457 | ENA

Stretch responsive miRNAs in human aortic valve interstitial cells

Project description:miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h. Six static control and six samples exposed to cyclic stretch 14% for 24h

2015-01-09 | E-GEOD-49558 | biostudies-arrayexpress

Distinct Gene Expression Patterns in Biomechanically Stretched Cardiomyocytes

Project description:Neonatal rat ventricular cardiomyocytes (NRVCMs) were stretched biaxially (112%/24h) or stimulated with phenylephrine (PE, 50 uM), both resulting in a similar degree of hypertrophy. Unstretched NRVCMs served as negative control. Affymetrix microarray analysis revealed 164 genes more than 2.0-fold up- and 21 genes less than 0.5-fold downregulated (p<0.01). Differential expression was confirmed by real-time PCR. Several genes of the “fetal gene program”, i.e. BNP (4.2-fold, all p<0.05) were induced by stretch as well as PE. We also verified the upregulation of known stretch-responsive genes, including HSP70 (20.9x) and c-myc (3.0x). Moreover, we identified genes exclusively induced by stretch, such as the cardioprotective and antihypertrophic cytokine GDF15 (24.8x) and the antihypertrophic factor heme oxygenase 1 (Hmox1, 10.8x; both confirmed on protein level). Of note, neither PE nor endothelin-1 were able to upregulate GDF15 and Hmox1, while angiotensin II significantly induced both genes. Conversely, addition of the AT1 receptor blocker irbesartan markedly blunted stretch-mediated GDF15 and Hmox1 induction, suggesting that the angiotensin II receptor mediates stretch-dependent signals. In conclusion, we report a comprehensive gene expression profile of cardiomyocytes subjected to biomechanical stress in comparison to pharmacologically induced hypertrophy. Our data imply that a stretch-specific gene program exists, that is mediated, at least in part, by angiotensin-II-dependent signalling. Keywords: stress response

2007-12-01 | GSE5996 | GEO

Transcription profiling of rat neonatal ventricular cardiomyocytes (NRVCMs) stretched biaxially (112%/24h) or stimulated with phenylephrine (PE, 50 uM

Project description:Neonatal rat ventricular cardiomyocytes (NRVCMs) were stretched biaxially (112%/24h) or stimulated with phenylephrine (PE, 50 uM), both resulting in a similar degree of hypertrophy. Unstretched NRVCMs served as negative control. Affymetrix microarray analysis revealed 164 genes more than 2.0-fold up- and 21 genes less than 0.5-fold downregulated (p<0.01). Differential expression was confirmed by real-time PCR. Several genes of the âfetal gene programâ, i.e. BNP (4.2-fold, all p<0.05) were induced by stretch as well as PE. We also verified the upregulation of known stretch-responsive genes, including HSP70 (20.9x) and c-myc (3.0x). Moreover, we identified genes exclusively induced by stretch, such as the cardioprotective and antihypertrophic cytokine GDF15 (24.8x) and the antihypertrophic factor heme oxygenase 1 (Hmox1, 10.8x; both confirmed on protein level). Of note, neither PE nor endothelin-1 were able to upregulate GDF15 and Hmox1, while angiotensin II significantly induced both genes. Conversely, addition of the AT1 receptor blocker irbesartan markedly blunted stretch-mediated GDF15 and Hmox1 induction, suggesting that the angiotensin II receptor mediates stretch-dependent signals. In conclusion, we report a comprehensive gene expression profile of cardiomyocytes subjected to biomechanical stress in comparison to pharmacologically induced hypertrophy. Our data imply that a stretch-specific gene program exists, that is mediated, at least in part, by angiotensin-II-dependent signalling. Experiment Overall Design: Three conditions were compared with two replicates each. These are: Experiment Overall Design: (1) control, i.e. no treatment; (2) induction by phenylephrine (50 uM); (3) induction by biomechanical stretch (112%/24h)

2008-06-14 | E-GEOD-5996 | biostudies-arrayexpress

Stretch responsive miRNAs in human aortic valve interstitial cells

Project description:miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h.

2015-01-09 | GSE49558 | GEO

The ion channel Trpc6a regulates the cardiomyocyte regenerative response to mechanical stretch

Project description:Myocardial damage caused for example by cardiac ischemia leads to ventricular volume overload resulting in increased stretch of the remaining myocardium. In adult mammals, these changes trigger an adaptive cardiomyocyte hypertrophic response which, if the damage is extensive, will ultimately lead to pathological hypertrophy and heart failure. Conversely, in response to extensive myocardial damage, cardiomyocytes in the adult zebrafish heart and neonatal mice proliferate and completely regenerate the damaged myocardium. We therefore hypothesized that in adult zebrafish, changes in mechanical loading due to myocardial damage may act as a trigger to induce cardiac regeneration. Based, on this notion we sought to identify mechanosensors which could be involved in detecting changes in mechanical loading and triggering regeneration. Here we show using a combination of knockout animals, RNAseq and in vitro assays that the mechanosensitive ion channel Trpc6a is required by cardiomyocytes for successful cardiac regeneration in adult zebrafish. Furthermore, using a cyclic cell stretch assay, we have determined that Trpc6a induces the expression of components of the AP1 transcription complex in response to mechanical stretch. Our data highlights how changes in mechanical forces due to myocardial damage can be detected by mechanosensors which in turn can trigger cardiac regeneration.

2023-12-20 | E-MTAB-13603 | biostudies-arrayexpress

Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

Project description:Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

| PRJNA421435 | ENA