Project description:The study examines transcriptomes of Trifolium pratense (red clover) roots challenged with the fungal pathogens Fusarium oxysporum and Phoma medicaginis. Fungal infection broadly activated both core metabolic processes and specialized metabolic pathways, including transcripts encoding phenylpropanoid and (iso)flavonoid biosynthetic enzymes. Among 33 predicted isoflavonoid-pathway genes, many were differentially expressed in at least one fungal treatment, with the late steps of pterocarpan biosynthesis particularly induced by P. medicaginis. These comparative transcriptomes provide insight into the activation and regulation of biotic stress responses as well as the engagement of legume-specific isoflavonoid biosynthesis, a pathway closely linked to antimicrobial defense.
Project description:Four new eremophilane sesquiterpenoids, together with five known analogues, were isolated from the marine-derived fungus Phoma sp. DXH009.
Project description:Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity
Project description:Phoma macdonaldii causes black stem in sunflower, which severely affects both the yield and quality of sunflowers including weight and oil content of seeds. In this study, a global proteomic analysis of P. macdonaldii was performed in order to facilitate a better understand of the biological and pathogenic characteristics of this fungus. A total of 1,498 proteins were identified by LC-MS/MS in all biological replicates. The identified proteins were classified into functional categories, which included biological process, cellular components and molecular function (GO analysis), metabolism, genetic information progressing, environmental information progressing, cellular processes and organismal systems (KEGG). In addition, the expression of eight genes associated with fungal development and pathogenicity was further investigated. To the best of our knowledge, this is the first study to characterize the proteome of P. macdonaldii. Results from this study provide novel insights into fungal development and pathogenesis of P. macdonaldii and, perhaps even other Phoma species and may contribute to an enhanced understanding the sunflower and P. macdonaldii pathosystem