Project description:Within this work we identified and characterized SMc03169 (hhrA) as a new Sinorhizobium meliloti gene product with relevance to biological nitrogen fixation symbiosis with leguminous plants. HhrA belongs to the TetR-family of repressors and its deletion from S. meliloti genome affected considerably gene expression as well as several phenotypic traits.
Project description:Single-cell RNA sequencing of Medicago truncatula roots was generated from nuclei using the 10X Genomics' Chromium technology. Roots were harvested and processed 24h after exposure to Ensifer meliloti (formerly Sinorhizobium meliloti).
Project description:We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA
Project description:Within this work we identified and characterized SMc03169 (hhrA) as a new Sinorhizobium meliloti gene product with relevance to biological nitrogen fixation symbiosis with leguminous plants. HhrA belongs to the TetR-family of repressors and its deletion from S. meliloti genome affected considerably gene expression as well as several phenotypic traits. For expression profiling, triplicates of the wild-type Sinorhizobium meliloti 1021 or the SMc03169 (delta hhrA) deletion mutant were grown in GMS liquid medium supplemented with 10 micromolar of luteolin for 4 hours at 30M-BM-:C and 180 rpm, followed by total RNA extraction.