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Maniola jurtina

ABSTRACT: Maniola jurtina Genome sequencing and assembly

PROVIDER: PRJNA498046 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR9707492_subreads.fastq.gz	Fastqsanger.gz
	SRR9707493_1.fastq.gz	Fastqsanger.gz
	SRR9707494_1.fastq.gz	Fastqsanger.gz
	SRR9707494_2.fastq.gz	Fastqsanger.gz

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De Novo Genome Assembly of the Meadow Brown Butterfly, Maniola jurtina.

Project description:Meadow brown butterflies (Maniola jurtina) on the Isles of Scilly represent an ideal model in which to dissect the links between genotype, phenotype and long-term patterns of selection in the wild - a largely unfulfilled but fundamental aim of modern biology. To meet this aim, a clear description of genotype is required. Here we present the draft genome sequence of M. jurtina to serve as a founding genetic resource for this species. Seven libraries were constructed using pooled DNA from five wild caught spotted females and sequenced using Illumina, PacBio RSII and MinION technology. A novel hybrid assembly approach was employed to generate a final assembly with an N50 of 214 kb (longest scaffold 2.9 Mb). The sequence assembly described here predicts a gene count of 36,294 and includes variants and gene duplicates from five genotypes. Core BUSCO (Benchmarking Universal Single-Copy Orthologs) gene sets of Arthropoda and Insecta recovered 90.5% and 88.7% complete and single-copy genes respectively. Comparisons with 17 other Lepidopteran species placed 86.5% of the assembled genes in orthogroups. Our results provide the first high-quality draft genome and annotation of the butterfly M. jurtina.

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