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RNA levels in wild-type and HIF1α-mutant MCF7 cells in normoxia (21% O2), and in hypoxia (1% O2) for 3 h or 24 h

ABSTRACT: RNA levels in wild-type and HIF1α-mutant MCF7 cells in normoxia (21% O2), and in hypoxia (1% O2) for 3 h or 24 h

PROVIDER: PRJNA503341 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8144260.fastq.gz	Fastqsanger.gz
	SRR8144261.fastq.gz	Fastqsanger.gz
	SRR8144252.fastq.gz	Fastqsanger.gz
	SRR8144262.fastq.gz	Fastqsanger.gz
	SRR8144253.fastq.gz	Fastqsanger.gz

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RNA levels in wild-type and HIF1α-mutant MCF7 cells in normoxia (21% O2), and in hypoxia (1% O2) for 3 h or 24 h

Project description:We report the generation of CRISPR/Cas9-engineered MCF7 human breast cancer cells that lack functional HIF1α. We show that the HIF1α-dependent transcriptional response to hypoxia is severely impaired in HIF1α-mutant MCF7 cells.

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Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)

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