Project description:Sacha Inchi (Plukenetia volubilis) oil press-cake (SIPC) represents a new source of proteins of high biological value, with promissory food applications. However, knowledge of these proteins remains limited. In this study, a Sacha Inchi protein concentrate (SPC) was extracted from the SIPC, and proteomic analysis was performed to identify the major alkaline-soluble proteins. Electrophoretic profiles highlighted the efficacy of conditions to extract the major proteins, from which five proteins, not previously reported, were registered. LC-MS/MS analyses produced abundant high-quality fragmentation spectra. Utilizing the Euphorbiaceae database (DB), 226 proteins were identified, with numerous well-assigned spectra remaining unidentified. The PEAKS Studio software generated high quality de novo peptides. Gene ontology (GO) classification allowed the identification of sequenced proteins associated with biological processes, molecular functions, and cellular components. The main alkali-soluble proteins from SPC were characterized by the derived functional analysis like seed storage, defense-related, and carbohydrate and lipid metabolism-related proteins. These results contribute to the information of the emerging proteomic studies on Sacha Inchi.
