Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tristetraprolin targets Nos2 expression in the colonic epithelium

ABSTRACT: Tristetraprolin targets Nos2 expression in the colonic epithelium

PROVIDER: PRJNA508390 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8281060.fastq.gz	Fastqsanger.gz
	SRR8281055.fastq.gz	Fastqsanger.gz
	SRR8281056.fastq.gz	Fastqsanger.gz
	SRR8281057.fastq.gz	Fastqsanger.gz
	SRR8281058.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Tristetraprolin targets Nos2 expression in the colonic epithelium

Project description:Tristetraprolin (TTP), encoded by the Zfp36 gene, is a zinc-finger protein that regulates RNA stability primarily through association with 3’ untranslated regions (3’UTRs) of target mRNAs. While TTP is expressed abundantly in the intestines, its function in intestinal epithelial cells (IECs) is unknown. Here we used a cre-lox system to remove Zfp36 in the mouse epithelium to uncover a role for TTP in IECs and to identify target genes in these cells. While TTP was largely dispensable for establishment and maintenance of the colonic epithelium, we found an expansion of the proliferative zone and an increase in goblet cell numbers in the colon crypts of Zfp36ΔIEC mice. Furthermore, through RNA-sequencing of transcripts isolated from the colons of Zfp36fl/fl and Zfp36ΔIEC mice, we found that expression of inducible nitric oxide synthase (iNos or Nos2) was elevated in TTP-knockout IECs. We demonstrate that TTP interacts with AU-rich elements in the Nos2 3’UTR and suppresses Nos2 expression. In comparison to control Zfp36fl/fl mice, Zfp36ΔIEC mice were less susceptible to dextran sodium sulfate (DSS)-induced acute colitis. Together, these results demonstrate that TTP targets Nos2 expression in IECs and aggravates acute colitis.

2019-12-04 | GSE123345 | GEO

Tristetraprolin targets Nos2 expression in the colonic epithelium.

Project description:Tristetraprolin (TTP), encoded by the Zfp36 gene, is a zinc-finger protein that regulates RNA stability primarily through association with 3' untranslated regions (3' UTRs) of target mRNAs. While TTP is expressed abundantly in the intestines, its function in intestinal epithelial cells (IECs) is unknown. Here we used a cre-lox system to remove Zfp36 in the mouse epithelium to uncover a role for TTP in IECs and to identify target genes in these cells. While TTP was largely dispensable for establishment and maintenance of the colonic epithelium, we found an expansion of the proliferative zone and an increase in goblet cell numbers in the colon crypts of Zfp36ΔIEC mice. Furthermore, through RNA-sequencing of transcripts isolated from the colons of Zfp36fl/fl and Zfp36ΔIEC mice, we found that expression of inducible nitric oxide synthase (iNos or Nos2) was elevated in TTP-knockout IECs. We demonstrate that TTP interacts with AU-rich elements in the Nos2 3' UTR and suppresses Nos2 expression. In comparison to control Zfp36fl/fl mice, Zfp36ΔIEC mice were less susceptible to dextran sodium sulfate (DSS)-induced acute colitis. Together, these results demonstrate that TTP in IECs targets Nos2 expression and aggravates acute colitis.

| S-EPMC6783411 | biostudies-literature

Colonic epithelium of WKY and SHR

Project description:Colonic epithelium of WKY and SHR

| PRJNA540986 | ENA

Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

Project description:Epithelia of small and large intestines differ in their structures and functions. Such heterogeneity between these two epithelial tissues might be controlled by both epithelium-intrinsic and -extrinsic programs. By employing the cell transplantation technique developed in our laboratory, we investigated how adult SI epithelial cells behave when heterotopically transplanted onto colon. Then the gene expression profiles of small intestinal epithelium heterotopically transplanted onto colon and control colonic epithelium were compared. We used laser capture microdissection and microarray analysis to compare the global gene expression profiles of small intestinal epithelium in the heterotopically grafted tissues and control colonic epithelium.

2014-08-15 | GSE59410 | GEO

ChIP-seq of Colonic Intestine Epithelium from Satb2-deficient and WT mice

Project description:We use ChIP-seq to understand the genomic binding sites of SATB2 in colonic epithelium and to study the shifting of CDX2 and HNF4A's genomic binding sites after SATB2 loss in colonic epithelium.

2021-10-05 | GSE167287 | GEO

Expression data from targeting of NOS2 in glioma stem cells

2011-07-08 | GSE29750 | GEO

Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

2014-08-15 | E-GEOD-59410 | biostudies-arrayexpress

Expression data from targeting of NOS2 in glioma stem cells

Project description:Analysis of the effects of targeting NOS2 at the gene expression level. Our studies demonstrated a role for NOS2 in glioma biology through the maintenance of the glioma stem cell phenotype. Microarray results provide novel targets of NOS2 and suggest mechanisms through which NOS2 contributes to glioma stem cell biology. Glioma stem cells isolated from two different human glioma xenografts were infected with a non-targeting control shRNA or two different shRNAs directed against NOS2 (each treatment in each tumor performed in technical duplicates).

2011-07-08 | E-GEOD-29750 | biostudies-arrayexpress

Bulk RNA-seq (>20M reads) of colonic epithelium from Vil-CreER;MTA2F/F (MTA2KO) and Wild Type mice

Project description:RNA-sequening colonic epithelium with deeper reads to evaluate consequence of mta2 gene loss in the colonic epithelium.

2024-03-07 | GSE213879 | GEO

Azoxymethane induced changes in Rat proximal and distal colonic epithelium.

Project description:Comparison of global gene expression in the proximal and distal colonic epithelium in azoxymethane treated rats. Keywords: disease state analysis

2008-12-05 | GSE13802 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data