Project description:In our study, we discovered that AQCA (Anthraquinone-2-carboxylic acid) effectively degrades Bacillus subtilis NCIB 3610 biofilms, a finding of significant research importance. To investigate the underlying mechanisms, we performed transcriptome sequencing of wild-type biofilms treated with either DMSO (control) or AQCA. Statistical analysis of gene expression (P < 0.05) revealed that AQCA treatment led to significant changes: 184 genes were upregulated, while 503 genes were downregulated by at least twofold compared to the DMSO control group.
Project description:The natural biotope of Bacillus subtilis is the upper layer of soil where it grows as a biofilm. To mimic this physiological development and study the impact of nanoparticles during the formation of a biofilm in a contaminated soil, we have studied the proteomic response of the ancestral strain Bacillus subtilis 3610, which is able to form biofilm contrary to the 168 laboratory strain. The bacteria were grown on soft agar plates containing n-ZnO, n-TiO2 or ZnSO4 metal ion.