Project description:ChIP followed by deep sequencing was performed with antibodies to ERalpha in U2OS-ERalpha cells treated with 17beta-estradiol. Examination of Eralpha binding sites in U2OS-Eralpha cells. Sequenced input was used as a control
Project description:To further characterize genomic regions previously identified which are linked with meat quality traits like drip loss, PH, conductivity and etc., we employed mRNA-seq experiment on the PiF1 animals used in the tiling array experiments. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Muscle mRNA profiles of 12 were generated by deep sequencing, in triplicate, using Illumina GAIIx.
Project description:ORCA is an ORC associated protein that plays important roles in replication initiation as well as heterochromatin organization. We carried out ORCA ChIP-seq in U2OS cells synchronized at different stage of G1 phase to determine its genome wide localization. To understand the genomic features of ORCA binding regions, we also carried out Methylated DNA IP (MeDIP) followed by deep sequencing in U2OS cells to determine the genome wide localizatoin of methyl-CpG sites in U2OS cells and how ORCA bidning regions co-localize with this important repressive mark.
Project description:Virtual memory CD8+ T (TVM) cells are a distinct subset of antigen-inexperienced cells with rapid effector potential, but their regulatory checkpoints remain elusive. Here, using DRIP sequencing, we found that loss of DUSP2 promotes aberrant R-loop accumulation, leading to enhanced chromatin accessibility and subsequent TVM cell differentiation.