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MRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)

ABSTRACT: mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)

PROVIDER: PRJNA513007 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8393570_1.fastq.gz	Fastqsanger.gz
	SRR8393570_2.fastq.gz	Fastqsanger.gz
	SRR8393571_1.fastq.gz	Fastqsanger.gz
	SRR8393571_2.fastq.gz	Fastqsanger.gz
	SRR8393566_1.fastq.gz	Fastqsanger.gz

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mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)

Project description:Purpose: PM2.5 can impact mouse TH17 cell differentitation. To Investigator the genes altered by PM2.5 during TH17 differentiation, mRNA-seq was performed. Methods: Naïve T cells were isolated from mouse spleen by magnetic beads. These naïve T cells were stimulated by CD3/CD28 antibodies and cultured under TH17 polarization conditions for 4 days before harvested. For PM2.5 group, 100ug/ml of PM2.5 suspensions were added to culture medium 6 hours after stimulation. The total RNA was extracted using the TRIzol reagent (Invitrogen). The library construction and sequencing was performed by Illumina Xten at Shanghai Biotechnology Corporation.

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