Project description:Purpose: The transcriptional alterations underlying physiological changes in fetal alcohol spectrum disorder are largely unidentified. Here we perform RNA sequencing on 0% and 1% EtOH (12 hpf - 5 dpf) zebrafish at 7dpf stages in order to identify significant transcriptional alterations.
Project description:The sclerotome region of the somite (labelled by nkx3.1:Gal4-VP16; UAS:NTR-mCherry) gives rise to numerous fibroblasts populations in the zebrafish trunk. We performed single cell RNA sequencing (scRNA-seq) on sclerotome-derived fibroblasts from 52 hpf embryos to determine population heterogeneity and plasticity.
Project description:In this study, we performed RNA-seq on 5 dpf livers of uhrf1-hi272 mutants and phenotypically wild-type siblings collected at 120 hpf.
Project description:This study was carried out to compare the changes in gene expression in 5 dpf zebrafish larval livers in response to mutation of the arsenic methylation gene (as3mt) - 8 bp deletion in exon 3 with and without 1 mM sodium arsenite treatment (96-120 hpf)
Project description:Quantitative proteomic data for 10 stages (including 4-cell, 256-cell, 1000-cell, Oblong, Dome, Bud, 16-hpf, 28-hpf, 3-dpf, 5-dpf) of early zebrafish development, each stage corresponds to two sets of biological repeats, and each set of biological repeats corresponds to two sets of technical repeats.
Project description:For this experiment, we analyzed gene expression changes and intron splicing events in the WT zebrafish intestine at two developmental stages (3 dpf and 5 dpf). To perform this analysis, we collected > 125 M paired end reads from total RNA isolated from purified cldn15la+ intestinal epithelial cells at each time point.