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Macrophage training to Borrelia burgdorferi governs cardiac inflammation in the mammalian host [RNA-seq]

ABSTRACT: Macrophage training to Borrelia burgdorferi governs cardiac inflammation in the mammalian host [RNA-seq]

PROVIDER: PRJNA516652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8477840.fastq.gz	Fastqsanger.gz
	SRR8477841.fastq.gz	Fastqsanger.gz
	SRR8477842.fastq.gz	Fastqsanger.gz
	SRR8477843.fastq.gz	Fastqsanger.gz
	SRR8477844.fastq.gz	Fastqsanger.gz

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Macrophage training to Borrelia burgdorferi governs cardiac inflammation in the mammalian host

Project description:Macrophage training to Borrelia burgdorferi governs cardiac inflammation in the mammalian host

| PRJNA516655 | ENA

Macrophage training to Borrelia burgdorferi governs cardiac inflammation in the mammalian host [RNA-seq]

Project description:Macrophage exposure to vaccine or vaccine-like formulations results in disparate secondary responses. We have identified the transcriptomic and metabolic changes associated with the memory response of macrophages to Borrelia burgdorferi, an extracellular pathogen able to establish long-term infections in mammals. Memory macrophages show an enhanced ability to bind and internalize the spirochete with decreased inflammatory responses, in part regulated by Irf4. Experienced macrophages also show an augmented glycolytic output. In vivo, glycolysis inhibition results in decreased cardiac inflammation and reduced expression of Irf4. Our data show that B. burgdorferi induces long-term, memory-like responses that are amenable to be manipulated in vivo.

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Project description:RNA was isolated from late-log pahse wild-type Borrelia burgdorferi B31 and the bb0647 mutant grown in BSKH media at 37 degree, 5% CO2. cDNA was synthesized, labeled and hybridized to the 70mer oligonucleotide B. burgdorferi array. Slides were scanned using axon scanner and image were analyzed using GenePix 6.0. Dta were further analysed using the professional software Acuity 4.o, based on a ratio-based normalization.

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