Project description:Time course intracellular proteomics of Streptomyces hygroscopicus NRRL 30439 (S. inhibens NRRL30439, CP084681) during submerged culture fermentation. Label free quantification of LC-MSMS spectra was used for protein expression profiling to support primary and secondary metabolic functionalities.

2025-06-03 | PXD030257 | Pride

Streptomyces achromogenes breed:Prokaryotic breed

Project description:Streptomyces achromogenes breed:Prokaryotic breed Genome sequencing and assembly

| PRJNA721982 | ENA

Ribosome pausing at the AT-rich codons regulates the protein expression of secondary metabolite gene clusters in the Streptomyces tsukubaensis NRRL 18488

Project description:Streptomyces tsukubaensis NRRL 18488 is the preferred strain for the production of immunosuppressant agent tacrolimus (FK506). To take full advantage of its genetic potential, systematic understanding of secondary metabolism and related regulatory mechanisms is highly demanded. Here, to this end, we complete its 7.9 Mbp linear genome sequence followed by integrating with multi-omics measurements. With accurate reannotation of FK506 gene cluster, total 2,389 transcription start sites were determined by using primary transcriptome analysis. Integrated analysis of transcriptome and translatome data revealed that secondary metabolic gene clusters, especially FK506 cluster, undergo translational control with decrease in translational efficiency according to the growth. Furthermore, we demonstrated that SD motif has little correlation with ribosome pausing but AT-rich codons delay the translational elongation. Strong ribosome pausing was observed in the rare TTA codon in FK506 cluster. This comprehensive genome-scale analysis provides insight to the translational regulation of secondary metabolism in S. tsukubaensis.

2019-10-16 | GSE97637 | GEO

Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine

Project description:Chitin is the second most abundant biopolymer present in soils and is utilized by antibiotic-producing Streptomyces species. Its monomer, N-acetylglucosamine (NAG), regulates the developmental program of the model organism Streptomyces coelicolor. NAG blocks differentiation when growing on rich medium whilst it promotes development on poor culture media. We report here the negative effect of NAG on tacrolimus (FK506) production in Streptomyces tsukubaensis NRRL 18488 growing on a defined rich medium. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, NAG stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis, pyruvate metabolism, and key gene members of the PHO regulon. The results obtained support those previously reported for S. coelicolor, but some important differences were observed

2020-02-08 | GSE110393 | GEO

Fusarium verticillioides strain:FGSC 7600 (M-3125 or NRRL 13447) and FGSC 7603

Project description:Fusarium verticillioides strain:FGSC 7600 (M-3125 or NRRL 13447) and FGSC 7603 Phenotype or Genotype

| PRJNA294283 | ENA

Klebsiella pneumoniae strain:3125

Project description:Klebsiella pneumoniae strain:3125 Genome sequencing and assembly

| PRJNA730878 | ENA

Helicobacter pylori strain:3125

Project description:Helicobacter pylori strain:3125 Genome sequencing and assembly

| PRJNA329327 | ENA