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RNA-seq using the Cel-Seq2 method, of wild type and 35-polyglutamine (polyQ35) expressing C. elegans worms treated with RNAi toward anc-1, or left untreated (EV) gene expression profiles.

ABSTRACT: RNA-seq using the Cel-Seq2 method, of wild type and 35-polyglutamine (polyQ35) expressing C. elegans worms treated with RNAi toward anc-1, or left untreated (EV) gene expression profiles.

PROVIDER: PRJNA522451 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8580020.fastq.gz	Fastqsanger.gz
	SRR8580030.fastq.gz	Fastqsanger.gz
	SRR8580021.fastq.gz	Fastqsanger.gz
	SRR8580031.fastq.gz	Fastqsanger.gz
	SRR8580022.fastq.gz	Fastqsanger.gz

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RNA-seq using the Cel-Seq2 method, of wild type and 35-polyglutamine (polyQ35) expressing C. elegans worms treated with RNAi toward anc-1, or left untreated (EV) gene expression profiles.

Project description:Purpose: We observed protein homeostasis modulations when anc-1 is knocked-down. We wanted to measure changes in gene expression profiles following this manipulation. Methods: We treated wild type (strain N2) or polyQ35-YFP (strain AM140) nematodes, which express toxic aggregative proteins that challenge their protein homeostasis, with anc-1 RNAi until day six of adulthood, and compared their gene expression levels to those of untreated worms. Results: The knockdown of anc-1 leads to modified expression levels of hundreds of genes. There is an enrichment of transcription factors and protein homeostasis modulators, such as E3 ubiquitin ligases. Conclusions: anc-1 regulates protection from toxic aggregative proteins, at least partially, by regulating the expression of genes that encode protein homeostasis factors.

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