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RNA sequencing of isogenic BRCA2 haploinsufficient vs. wild-type T-ALL cells

ABSTRACT: RNA sequencing of isogenic BRCA2 haploinsufficient vs. wild-type T-ALL cells

PROVIDER: PRJNA523250 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8592570_1.fastq.gz	Fastqsanger.gz
	SRR8592570_2.fastq.gz	Fastqsanger.gz
	SRR8592580_1.fastq.gz	Fastqsanger.gz
	SRR8592580_2.fastq.gz	Fastqsanger.gz
	SRR8592571_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of isogenic BRCA2 haploinsufficient vs. wild-type T-ALL cells

Project description:We found a high frequency of heterozygous Fanconi-BRCA pathway mutations in pediatric T-ALL. BRCA2 was the most commonly mutated gene. We transduced Cas9-expressing Jurkat cells, which lacked an identifiable BRCA2 mutation, with an integration-defective lentiviral guide RNA expression construct targeting exon 11 of BRCA2 (NM_000059). Single-cell cloning and sequencing analysis revealed two distinct clones harboring monoallelic BRCA2 frameshift mutations, termed clones W4 and W5. Each of these clones was subjected to RNA sequencing analysis.

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