Project description:Flowering of perennial Arabis alpina, is differentially regulated on primary and axillary shoots. Although contributions of vernalization and aging pathways have been analyzed, those of photoperiodic flowering genes CONSTANS (CO), FLOWERING LOCUS T (FT), and TWIN-SISTER OF FT (TSF) remain unexplored. CRISPR-Cas9 mutations in AaCO and AaFT/TWIN SISTER OF FT-LIKE (TSFL) were recovered. Aaco and Aaft/tsfl mutants in pep1 background were scored for flowering time, inflorescence branching and floral phenotypes under long (LD) or short days (SD) and after vernalization. RNAseq data on primary and axillary branches were compared. AaCO activates AaFT/TSFL transcription in leaves, and Aaco and Aaft tsfl mutations delay flowering under LDs. Axillary branches flowered in Aaco mutants but not in Aaft tsfl mutants. Both lacked inflorescence branches and flowers on the primary shoot under LDs. However, Aaft tsfl mutants produced some flowers after vernalization, and Aaco a few in SDs. RNAseq identified genes responsive to AaFT, TSFL and AaCO on the primary shoot and in axillary branches. Therefore, AaCO-AaFT promote flowering of A. alpina under LDs, but with distinct roles in axillary branch flowering. Both genes are required for inflorescence branching and flower formation on the primary shoot. The complex role of the CO-FT module in inflorescence architecture may underlie the polycarpic, perennial life history of A. alpina.
Project description:To investigate the cooperation of light drought with flower branches building and flower bud formation in Citrus, we set two groups including 75 days light drought (LD) and CK to uncover which target genes relatived to flower bud and branches building have been influenced by LD. We then performed gene expression profiling analysis using data obtained from RNA-seq of two different treatment.
Project description:To identify candidate genes involved in maturation and flowering, we conducted microarray expression studies using two poplar genotypes (Populus trichocarpa x P. deltoides hybrids) represented in continuous age gradients of one to six years. We designed 70-mers for 228 poplar genes and microarray studies were carried out using the microplate-based 96-well BioGridArray platform (GeneXP Biosciences). Floral buds, vegetative buds and shoot tips were collected at different seasonal time points from juvenile and adult trees and from both basal and upper branches of mature trees. Keywords: Maturation and Flowering
Project description:Early-flowering germplasm XH04 (E) and late-flowering germplasm XH05 (L) were screened. Panicle samples were collected at booting stage (B), heading stage (H) and flowering stage (F) for proteome sequencing