Circadian transcriptome in mouse skeletal muscle after exercise in the early rest versus active phase
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA523847 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8611760_1.fastq.gz | Fastqsanger.gz | |||
| SRR8611760_2.fastq.gz | Fastqsanger.gz | |||
| SRR8611770_1.fastq.gz | Fastqsanger.gz | |||
| SRR8611770_2.fastq.gz | Fastqsanger.gz | |||
| SRR8611780_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 280 |
Similar Datasets
Project description:We performed the circadian transcriptome analysis using the skeletal muscle from sedentary and exercised mice either in the early rest phase (ZT3) or in the early active phase (ZT15). By the combination with circadian transcriptomic and metabolomic analysis, we revealed time-of-day-dependent remodeling of circadian muscular metabolic pathways involved in glucose and glycerol metabolism after exercise. We found that only exercise in the early active phase elevates the levels of genes encoding glycolytic enzymes followed by the activation of fatty acid oxidation, branched-chain amino acid catabolism and ketogenesis/ketosis. This study demonstrates that time-of-day is a critical factor to modulate the impact of exercise on metabolic pathways within skeletal muscle.
2019-04-01 | GSE126962 | GEO
Project description:The regulation of metabolism in peripheral tissues is mediated by pathways that are under circadian control. a bidirectional relationship is evident between the circadian clock and hypoxia-inducible factor-1 α (HIF1α), which is specific to time-of-day exercise. To elucidate the role of HIF1α in driving the divergences in the systemic and skeletal muscle metabolic responses to exercise, a skeletal muscle-specific HIF1α knockout (KO) mouse model was generated. Metabolic phenotyping alongside global transcriptomic profiling was conducted under basal conditions or in response to an acute exercise bout in HIF1α KO and wild-type (WT) mice at the early rest phase (ZT3) or early active phase (ZT15). Our findings demonstrate that HIF1α is dispensable for the metabolic response to exercise at ZT15, but required for an elevation in glycolytic metabolism in response to exercise at ZT3. Specifically, the loss of HIF1α alters the fate of glucose with a shift towards mannose-6-phosphate production observed at ZT3. In addition, the loss of HIF1α alters the transcriptional profile of skeletal muscle specifically at ZT3 with a shift towards oxidative metabolism. HIF1α contributes to regulating the time-of-day exercise response by driving a shift in substrate preference primarily towards glucose oxidation while sparing fatty acid oxidation. This comprehensive metabolic phenotyping of HIF1α KO mice demonstrates that the contribution of HIF1α to the regulation of time-of-day metabolism is dependent on metabolic status and energetic stressors.
2025-06-21 | GSE282641 | GEO
Project description:Unconditioned thoroughbred geldings were exercised to maximal heart rate or fatigue on an equine high-speed treadmill. Skeletal muscle biopsies were taken from the middle gluteal muscle before, immediately after and four hours after exercise. Three-condition experiment, Pre exercise (T0), Immediately post exercise (T1), 4 hours post exercise (T2). Hybridisations: T0 vs T1, T0 vs T2 Biological replicates: 8 Technical replication Dye swap
2010-06-21 | E-GEOD-16235 | biostudies-arrayexpress
Project description:Unconditioned thoroughbred geldings were exercised to maximal heart rate or fatigue on an equine high-speed treadmill. Skeletal muscle biopsies were taken from the middle gluteal muscle before, immediately after and four hours after exercise.
2009-10-14 | GSE16235 | GEO
Project description:The participants performed 8 weeks of superised aerobic endurance exercise. Skeletal muscle biopsise were taken at rest before and after intervention and matched analysis was performed.
2024-04-24 | GSE244359 | GEO
Project description:Microgravity has a dramatic impact on human physiology, illustrated in particular with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for the definition of efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n=8), and the potential beneficial impact of protein supplementation (BRN group, n=8) and of a combined resistance and aerobic training (BRE group, n=8). Gene expression profiles were obtained using an in-house made microarray containing 6681 muscles-relevant genes. A two-class statistical analysis was applied on the 2103 genes with consolidated expression. We identified 472 and 207 modified genes, respectively for SOL and VL in BRC group. Further clustering approaches, identifying relevant biological mechanisms or pathways, underlined five main subclusters. Three are composed almost of upregulated genes involved mainly in nucleic acid and protein metabolism, and two composed almost of downregulated genes involved in energy metabolism. Exercise countermeasure demonstrated a drastic compensatory effect, decreasing the number of differentially-expressed genes by 89 and 96% in SOL and VL. In contrast, nutrition countermeasure had a moderate effect and decreased the number of differentially-expressed genes by 40 and 25% in SOL and VL. Our results allowed reporting a systematic, global and comprehensive view of long-term woman muscle atrophy and brought new lights and insights for space environment and for women who undergo a long-term clinical bed rest. Biological samples were collected from Pre- and Post- bed rest (BR) soleus and vastus lateralis biopsies of each subject from the three groups (bed rest only: BRC; Exercise: BRE; Nutrition: BRN). six technical replicate values (2 duplicate hybridizations âa et bâ to chips with triplicate spots âxxxâ) were obtained for each skeletal muscle sample. Thus for each subject, 12 expression measurements (6 before BR and 6 after BR) were obtained for each muscle.
2010-09-10 | E-GEOD-14798 | biostudies-arrayexpress
Project description:The circadian clock is a cell-autonomous transcription-translation feedback mechanism that anticipates and adapts physiology and behavior to different phases of the day. A variety of factors including hormones, temperature, food-intake, and exercise can act on tissue-specific peripheral clocks to alter the expression of genes that influence metabolism, all in a time-of-day dependent manner. The aim of this study was to elucidate the effects of exercise timing on adipose tissue metabolism. We performed RNA sequencing on inguinal adipose tissue of mice immediately following maximal exercise or sham treatment at the early rest or early active phase. Only during the early active phase did exercise elicit an immediate increase in serum non-esterified fatty acids. Furthermore, early active phase exercise increased expression of markers of thermogenesis and mitochondrial proliferation in inguinal adipose tissue. In vitro, synchronized 3T3-L1 adipocytes showed a timing-dependent difference in Adrb2 expression, as well as a greater lipolytic activity. Thus, the response of adipose tissue to exercise is time- of-day sensitive and may be partly driven by the circadian clock. To determine the influence of feeding state on the time-of-day response to exercise, we replicated the experiment in 10- hour-fasted early rest phase mice to mimic the early active phase metabolic status. A 10-hour fast led to a similar lipolytic response as observed after active phase exercise, but did not replicate the transcriptomic response, suggesting that the observed changes in gene expression are not driven by feeding status. In conclusion, acute exercise elicits timing-specific effects on adipose tissue to maintain metabolic homeostasis.
2023-01-19 | GSE199429 | GEO
Project description:Four healthy human volunteers underwent an acute bout of resistance exercise with the right leg at 2 pm. Biopsies were removed from the Vastus Lateralis muscle 6 h (8 pm) and 18 h (8 am) after exericise Keywords = Human skeletal muscle Keywords = resistance exerise Keywords = diurnal Keywords = circadian Keywords: time-course
2004-10-14 | GSE1832 | GEO
Project description:Timed physical activity might potentiate the health benefits of training. The underlying signaling events triggered by exercise at different times of the day are, however, poorly understood. Here, we found that time-dependent variations in maximal treadmill exercise capacity of naïve mice were associated with energy stores, mostly hepatic glycogen levels. Importantly, running at different times of the day resulted in a vastly different activation of signaling pathways, e.g., related to stress response, vesicular trafficking, repair, and regeneration. Second, voluntary wheel running at the opposite phase of the dark, feeding period surprisingly revealed minimal Zeitgeber (i.e., synchronizing) activity of training. This integrated study provides important insights into the circadian regulation of endurance performance and the control of the circadian clock by exercise. In future studies, these results could contribute to better understand circadian aspects of training design in athletes and the application of chrono-exercise-based interventions in patients.
2021-06-22 | PXD026461 | Pride
Project description:In this study, the properties of circulating EVs were examined in cerebral palsy (CP) and typically developed (TD) individuals at rest and after aerobic exercise. We performed smallRNA-seq anf focused on the microRNA cargo of EVs
2022-12-10 | GSE218103 | GEO