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Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging

ABSTRACT: Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging

PROVIDER: PRJNA529060 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8777204_1.fastq.gz	Fastqsanger.gz
	SRR8777204_2.fastq.gz	Fastqsanger.gz
	SRR8777205_1.fastq.gz	Fastqsanger.gz
	SRR8777205_2.fastq.gz	Fastqsanger.gz
	SRR8777206_1.fastq.gz	Fastqsanger.gz

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Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [SFO]

Project description:We performed RNAseq analysis to determine the effect of MFN2 deletion on oocyte global gene expression profile. RNAseq revealed a total of 1041 genes were significantly differentially expressed (p<0.05) in Mfn2-/- seceondary follicle enclosed oocytes (SFOs) compared to WT, with 510 up-regulated and 531 down-regulated genes. GO cluster analysis indicated significant over-representation of elements involved in regulation of cell death and survival, cellular development and cellular growth. Pathway analysis of top10 upregulated and downregulated annotated genes are listed. Our findings provide new insight into the role of MFN2 in the oocytes, and may help understand the potential mechanism of subfertility and reproductive aging associated with MFN2-deficiency.

2019-09-30 | GSE128808 | GEO

Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [GV]

Project description:Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [GV]

| PRJNA528880 | ENA

Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [SFO]

Project description:Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [SFO]

| PRJNA528927 | ENA

Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [GV]

Project description:We performed RNAseq analysis to determine the effect of MFN2 deletion on oocyte global gene expression profile. RNAseq revealed a total of 363 genes were significantly differentially expressed (p<0.05) in Mfn2-/- GV oocytes compared to WT (241 up-regulated and 122 down-regulated). Gene ontology (GO) analysis indicated significant over-representation of elements involved in regulation of embryonic development, cell death and survival, and cell morphology. Pathway analysis of top10 upregulated and downregulated annotated genes are listed. Our findings provide new insight into the role of MFN2 in the oocytes, and may help understand the potential mechanism of subfertility and reproductive aging associated with MFN2-deficiency.

2019-09-30 | GSE128780 | GEO

Mitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging.

Project description:Mitochondria change their shape through fusion and fission in order to adapt to their metabolic milieu. Mitofusin-2 (MFN2) is a key regulatory protein in this process, mediating mitochondrial fusion and interaction with endoplasmic reticulum. Targeted deletion of Mfn2 in oocytes resulted in mitochondrial dysfunction and female subfertility associated with impaired oocyte maturation and follicle development. Oocytes lacking MFN2 showed shortened telomeres and increased apoptosis, resulting in compromised oocyte quality and accelerated follicular depletion, consistent with a reproductive aging phenotype.

| S-EPMC6628992 | biostudies-literature

Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-09-30 | GSE128843 | GEO

Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve.

Project description:Mitochondria are dynamic organelles that continually adapt their structure through fusion and fission in response to changes in their bioenergetic environment. Targeted deletion of mitochondrial fusion protein mitofusin1 (MFN1) in oocytes resulted in female infertility associated with failure to achieve oocyte maturation. Oocyte-granulosa cell communication was impaired, and cadherins and connexins were downregulated, resulting in follicle developmental arrest at the secondary follicle stage. Deletion of MFN1 in oocytes resulted in mitochondrial dysfunction and altered mitochondrial dynamics, as well as accumulation of ceramide, which contributed to increased apoptosis and a reproductive phenotype that was partially rescued by treatment with ceramide synthesis inhibitor myriocin. Absence of MFN1 and resulting apoptotic cell loss also caused depletion of ovarian follicular reserve, and a phenotype consistent with accelerated female reproductive aging.

| S-EPMC6646343 | biostudies-literature

Vitrification preserves oocyte transcriptome in a 3D in vitro follicle development and oocyte maturation system

Project description:Vitrification is increasingly used to cryopreserve gametes and embryos in assisted reproductive technology (ART). Our prior research demonstrates that vitrification preserves the viability and functionality of ovarian follicles. However, its impact on follicle-enclosed oocyte remains unknown. The current study investigates whether vitrification, combined with a 3D encapsulated in vitro follicle growth (eIVFG) system, maintains oocyte transcriptome during in vitro follicle development and oocyte maturation. Immature mouse preantral follicles were vitrified and cultured in eIVFG for 8 days to grow to the preovulatory stage, followed with the induction of ovulation and oocyte maturation on day 9, with fresh follicles as the control. Oocytes at germinal vesicle (GV) stage from grown preovulatory follicles on day 8 and oocytes at metaphase II (MII) upon ovulation on day 9 were collected for single-oocyte Smart-Seq2 RNA sequencing analysis. The principal component analysis (PCA) separated GV and MII oocytes into two distinct clusters, but oocytes from fresh and vitrified follicles were largely overlapped. Differential gene expression (DEG) analysis revealed that GV or MII oocytes from fresh and vitrified follicles had comparable expression of maternal effect genes and other genes related to oocyte meiotic and developmental competence. There was a significant transcriptomic change during the GV-to-MII transition. Gene ontology (GO) and KEGG analysis identified DEGs between GV and MII oocytes related to cell cycle, RNA processing, mitochondria, and ribosome. In summary, our study demonstrates that vitrification preserves oocyte transcriptome during in vitro follicle development and oocyte maturation, supporting its potential in fertility preservation. Moreover, our single-oocyte RNA sequencing analysis identifies key DEGs upon GV-to-MII transition, indicating their potential functions in underpinning oocyte meiotic and developmental competence.

2025-04-02 | GSE292062 | GEO

Rejuvenation of aged oocyte through exposure to young follicular microenvironment

Project description:Reproductive aging is a major cause of fertility decline, attributed to decreased oocyte quantity and competence. Follicular somatic cells play crucial roles in the growth and development of the oocyte by providing nutrients and regulatory factors. Here we investigated how oocyte quality is affected by its somatic cell environment by creating chimeric follicles, whereby an oocyte from one follicle was transplanted into and cultured within another follicle whose native oocyte was removed. Somatic cells within the chimeric follicle re-establish connections with the oocyte and support oocyte growth and maturation in a three-dimensional (3D) culture system. We show that young oocytes transplanted into aged follicles exhibited reduced meiotic maturation and developmental potential, whereas the young follicular environment significantly improved the rates of maturation, blastocyst formation and live birth of aged oocytes. Aged oocytes cultured within young follicles exhibited enhanced interaction with somatic cells, more youth-like transcriptome, remodelled metabolome, improved mitochondrial function, and enhanced fidelity of meiotic chromosome segregation. These findings provide the basis for a future follicular somatic cell-based therapy to treat age-associated female infertility.

2024-07-22 | GSE270016 | GEO

Transcription profiling of mouse oocyte at primary follicle stage

Project description:oocytes were collected from ovarys of 6 day old female mice. Targets from four biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430 A & B. Experiment Overall Design: 4 oocyte at primary follicle stage biological replicates were analyzed

2008-06-12 | E-GEOD-3291 | biostudies-arrayexpress

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