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Defining DC response to cognate T cell interaction in the antigen-challenged lymph node [dataset 3]

ABSTRACT: Defining DC response to cognate T cell interaction in the antigen-challenged lymph node [dataset 3]

PROVIDER: PRJNA530542 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8836840.fastq.gz	Fastqsanger.gz
	SRR8836850.fastq.gz	Fastqsanger.gz
	SRR8836860.fastq.gz	Fastqsanger.gz
	SRR8836870.fastq.gz	Fastqsanger.gz
	SRR8836841.fastq.gz	Fastqsanger.gz

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Defining DC response to cognate T cell interaction in the antigen-challenged lymph node [dataset 3]

Project description:We designed an experimental set up to probe DC responses to cognate T cell encounter in antigen draining LN

2019-04-03 | GSE129213 | GEO

DC Respond to Cognate T Cell Interaction in the Antigen-Challenged Lymph Node.

Project description:Dendritic cells (DC) are unrivaled in their potential to prime naive T cells by presenting antigen and providing costimulation. DC are furthermore believed to decode antigen context by virtue of pattern recognition receptors and to polarize T cells through cytokine secretion toward distinct effector functions. Diverse polarized T helper (TH) cells have been explored in great detail. In contrast, studies of instructing DC have to date largely been restricted to in vitro settings or adoptively transferred DC. Here we report efforts to unravel the DC response to cognate T cell encounter in antigen-challenged lymph nodes (LN). Mice engrafted with antigen-specific T cells were immunized with nanoparticles (NP) entrapping adjuvants and absorbed with antigen to study the immediate DC response to T cell encounter using bulk and single cell RNA-seq profiling. NP induced robust antigen-specific TH1 cell responses with minimal bystander activation. Fluorescent-labeled NP allowed identification of antigen-carrying DC and focus on transcriptional changes in DC that encounter T cells. Our results support the existence of a bi-directional crosstalk between DC and T cells that promotes TH1 responses, including involvement of the ubiquitin-like molecule Isg15 that merits further study.

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Cognate T cell interactions affect the DC transcriptome [dataset 4]

Project description:Cognate T cell interactions affect the DC transcriptome [dataset 4]

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Cognate T cell interactions affect the DC transcriptome [dataset 4]

Project description:Our data suggest that peptide-presenting DC receive signals from synapse-forming antigen specific CD4+ or CD8+ T cells that alter the DC expression profile. To further test this notion, we hock immunized T cell-engrafted mice with NP harboring CpG and coated with OVA (OVA / CpG NP) or the control antigen BSA (BSA / CpG NP) and isolated NP+ DC for RNAseq.

2019-04-03 | GSE129214 | GEO

Human melanoma-derived extracellular vesicles reprogram dendritic cells potentially enabling lymph node metastases

Project description:Evolution of melanoma from a primary tumor to widespread metastasis is crucially dependent on lymphatic spread. The mechanisms regulating the initial step in metastatic dissemination via regional lymph nodes remain largely unknown. We have previously described a dysfunctional immune profile that precedes evidence of metastasis in the first node draining from the primary tumor, the sentinel lymph node (SLN). Herein, we explore the role of melanoma-derived extracellular vesicles (EVs) as mediators of this pre-metastatic niche through cargo-specific polarization of dendritic cells (DCs). Utilizing mass cytometry, pre-metastatic SLNs demonstrate compromised co-stimulatory CD80 expression compared to healthy lymph nodes. Similarly, DCs matured in vitro in the presence of melanoma EVs showed impaired co-stimulation and polarization towards a chronic inflammatory cytokine milieu. Profiling of melanoma EV cargo identified shared proteomic and RNA signatures including the signaling axis S100A8, S100A9 and cognate receptor TLR4. Mechanistically, S100A8 and S100A9 compromised DC maturation, a phenotype which was partially recovered following TLR4 blockade. Early evidence demonstrates similar EVs can be isolated from human afferent lymphatic fluid ex vivo. Taken together, we propose synergistic interactions among melanoma EV cargo are responsible for suppressing DC maturation, potentially explaining the survival of malignant melanocytes metastasizing into seemingly “normal” regional lymph nodes.

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Optineurin inhibition impedes type II collagen challenged dendritic cell migration

Project description:Due to the maturation, migration and antigen presentation functions, dendritic cells (DCs) play a central role in the development of many autoimmune diseases, like rheumatoid arthritis, multiple sclerosis and dermatitis. However, the molecular regulatory process of disease- or antigen-specific DC activation remains elusive. We show that deletion of Optineurin (OPTN) effectively impairs type II collagen (CII), the main autoantigen for rheumatoid arthritis, challenged DC migration, thus ameliorating collagen-induced arthritis. Transcriptome analyses indicate that OPTN promotes the expressions of migration related genes. Our findings thus indicate that OPTN is an important regulator of CII-pulsed DC migration, which may be of potential value for the accurate treatment of rheumatoid arthritis.

2025-05-22 | GSE240706 | GEO

Armatimonadetes bacterium DC isolate:DC

Project description:Armatimonadetes bacterium DC isolate:DC Genome sequencing and assembly

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Vitamin A treatment rescues neonatal infection-induced durably impaired lymph node function (RNA-Seq DC)

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Experimental challenge with bovine herpesvirus 1 in dairy calves: bronchial lymph node mRNA transcriptome response

Project description:Bovine Herpesvirus 1 (BoHV1) is a leading cause of Bovine Respiratory Disease (BRD) in young calves, which is responsible for substantial morbidity and mortality. Therefore, the objective of the current study was to elucidate the bronchial lymph node mRNA transcriptomic response to an experimental challenge with BoHV1, in dairy calves. Holstein-Friesian calves were either challenged by intranasal atomisation with BoHV1 virus (6.3 x 10^7/mL x 1.35mL) (n=12) or mock challenged with sterile phosphate buffered saline (n=6). Clinical signs were scored daily until euthanasia at day 6 post-challenge. Total RNA was extracted and sequenced from bronchial lymph node tissue (150 bp paired-end). Sequence reads were aligned to the ARS-UCD1.2 bovine reference genome and differential gene expression analysis was performed using EdgeR. An MDS plot displayed an obvious separation between BoHV1 challenged and control calves based on bronchial lymph node gene expression changes. There were 337 differentially expressed (DE) genes (p < 0.05, FDR < 0.1, fold change > 2) between the BoHV1challenged and control calves.

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