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Hematopoietic stem cells in perisinusoidal niches are protected from ageing

ABSTRACT: Hematopoietic stem cells in perisinusoidal niches are protected from ageing

PROVIDER: PRJNA535426 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8889320_1.fastq.gz	Fastqsanger.gz
	SRR8889320_2.fastq.gz	Fastqsanger.gz
	SRR8889321_1.fastq.gz	Fastqsanger.gz
	SRR8889321_2.fastq.gz	Fastqsanger.gz
	SRR8889322_1.fastq.gz	Fastqsanger.gz

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

Project description:With ageing, intrinsic hematopoietic stem cell (HSC) activity decreases, resulting in impaired tissue homeostasis, reduced engraftment following transplantation and increased susceptibility to diseases. However, whether ageing affects also the HSC niche impairing the capacity to support HSC function is still largely unknown. Here, by using in-vivo long-term label retention assays we demonstrate that aged labelling retaining (LR) HSCs, which are in the old mice the most quiescent HSC subpopulation with the highest regenerative capacity and cellular polarity, reside predominantly in perisinusoidal niches. Furthermore, we demonstrate that sinusoidal niches and perisinusoidal Nes-GFPlow cells are uniquely preserved in shape, morphology and number upon ageing. Finally, we show that myeloablative chemotherapy can selectively disrupt aged sinusoidal niches, which is linked to hematopoietic failure and decreased survival of aged mice. Overall, our data characterize for the first time the functional alterations of the aged HSC niche and unveil that perisinusoidal niches are uniquely preserved and protect HSCs from ageing.

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [young and aged BM CD45-CD31+ endothelial cells]

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

Project description:Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [young and aged BM CD45-CD31+ endothelial cells]

Project description:Hematopoietic stem cells in perisinusoidal niches are protected from ageing [young and aged BM CD45-CD31+ endothelial cells]

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Ageing-associated proteome and acetylome of hematopoietic progenitor cells

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing

Project description:This SuperSeries is composed of the SubSeries listed below.

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Tet2 deficiency altered hematopoietic stem and progenitor cells ageing process

Project description:Tet2 deficiency altered hematopoietic stem and progenitor cells ageing process

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single cell RNA sequencing of hematopoietic stem cells aged in old and young niches

Project description:Purpose: Compare the transcriptome of hematopoietic stem cells (HSCs) that were aged in old and young niches Methods: barcoded GFP+ HSCs were FACS-sorted from a) three recipient mice 15 months post transplantation, and b) six serial transplantation recipient mice 5 months after the 8th transplantation, then subjected to processed using the Chromium Single-cell 3′ v2 Library Kit (10× Genomics, Pleasanton, CA) following the manufacturer’s instructions Results: we obtained transcriptomes of about 12k HSCs aged in young niche, and about 10k HSCs aged in old niche, with the average sequencing depth at close to 50k reads per cell Conclusions: we identified striking differences in gene expression profiles 1) between HSCs aged in young niches from mice with early aging and from mice with delayed aging, and 2) between HSCs aged in old niches and young niches when mice exhibited hematopoietic aging phenotype

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