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Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function

ABSTRACT: Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function

PROVIDER: PRJNA540676 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8997060_1.fastq.gz	Fastqsanger.gz
	SRR8997060_2.fastq.gz	Fastqsanger.gz
	SRR8997070.fastq.gz	Fastqsanger.gz
	SRR8997080.fastq.gz	Fastqsanger.gz
	SRR8997090.fastq.gz	Fastqsanger.gz

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Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function [RRBS]

Project description:Transcriptomic and genome-wide DNA methylation was performed on T helper cells using RNA-sequencing and Reduced Representation Bisulfite Sequencing, respectively. Specific genome-wide DNA methylation analysis of T helper cells from women with PCOS identified 5,581 differentially methylated CpG sites. Interestingly, functional gene ontology enrichment analysis showed that genes located at the proximity of differentially methylated CpG sites belong to pathways related to reproductive function and immune cell function. However, these genes were not altered at the transcriptomic level. Our results show that PCOS is associated with global and gene-specific DNA methylation remodelling in a cell-type specific manner.

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Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function [RNAseq]

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Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function [RRBS]

Project description:Epigenetic reprogramming of immune cells in women with PCOS impact genes controlling reproductive function [RRBS]

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Proteome of follicular Fluid from lean and overweight/obese women with PCOS and that of healthy controls

Project description:Polycystic ovary syndrome (PCOS) is typically characterized by oligo or anovulation, hyperandrogenism and polycystic ovarian morphology, affecting 5-20% of women of reproductive age [1]. It has drawn significant attention as a major cause of anovulatory infertility and the syndrome of metabolic, reproductive and obstetrical disorders [2 ,+]. Due to heterogeneous clinical features and unclear pathogenesis of PCOS, the diagnosis and treatment strategies remain a matter of debate. To better understand the complex follicular development environment in PCOS, we conducted a TMT-based quantitative proteomic study to compare the composition of proteins, pathways and molecular functions of FF from lean and overweight/obese women with PCOS and that of healthy controls.

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Project description:Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder affecting women of reproductive age. The main features of PCOS are hyperandrogenism and irregular menstrual cycles together with metabolic dysfunctions including abdominal obesity, dyslipidemia and an increased risk of developing type 2 diabetes. Despite the high prevalence of >15%, the pathophysiology of the syndrome is unclear. Gene expression array data from skeletal muscle and adipose tissue have provided some information about dysregulated metabolic pathways in women with PCOS, but the transcriptomic data need to be verified by proteomics to advance our understanding of PCOS. Skeletal muscle and adipose tissue biopsies from 10 women with PCOS and 10 controls were subjected to global proteomic analysis. Protein expression differences between cases and controls were based on Student’s t-test and corrected for multiple testing. In total, we identified 5000 proteins in adipose tissue and 3480 proteins in skeletal muscle. After correction for multiple testing, 74 proteins with q < 0.05 corresponding to 72 unique proteins were found to be differentially expressed in adipose tissue from women with PCOS versus controls. And, 123 proteins with q < 0.05 corresponding to 120 unique proteins were found to be differentially expressed in skeletal muscle from women with PCOS versus control. We then applied pathway analysis to the total protein and phosphopeptide data using PRISM and Enrichr.

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