Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of manually sorted transgenically labeled sNPFr-Gal4 and TRH-Gal4 drosphila neurons

ABSTRACT: RNA-seq of manually sorted transgenically labeled sNPFr-Gal4 and TRH-Gal4 drosphila neurons

PROVIDER: PRJNA543486 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9080340.fastq.gz	Fastqsanger.gz
	SRR9080350.fastq.gz	Fastqsanger.gz
	SRR9080360.fastq.gz	Fastqsanger.gz
	SRR9080370.fastq.gz	Fastqsanger.gz
	SRR9080380.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

RNA-seq of manually sorted transgenically labeled sNPFr-Gal4 and TRH-Gal4 drosphila neurons

Project description:RNA-seq was used to molecularly characterize two drosophila neuron subtypes with demonstrated roles in the modulation of aggression

2019-05-18 | GSE131405 | GEO

Transcriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytes

Project description:Transcriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytes

| PRJNA940089 | ENA

Gene expression profile of Tra1 GID (Gal4 interaction defective) mutants

Project description:Promoter-specific transcriptional activators (activators) stimulate transcription through direct interactions with one or more components of the transcription machinery, termed the “target”. Previous studies have provided evidence that the Tra1 subunit of the yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) complex is the target of the yeast activator Gal4. However, several other general transcription factors, in particular the mediator complex, have also been implicated as Gal4 targets. To investigate the essentiality of Tra1 as a target of Gal4, here we derive Tra1 mutants that are selectively defective for interaction with Gal4 in vivo (Gal4 Interaction Defective (GID) mutants). In contrast to wild-type Tra1, Tra1 GID mutants are not recruited by Gal4 to the promoter and cannot support Gal4-directed transcription activation, demonstrating that the Gal4–Tra1 interaction is required for Gal4 function. In yeast strains expressing a Tra1 GID mutant, Gal4 promoter binding is unexpectedly also diminished indicating that Gal4 and Tra1 bind cooperatively. Consistent with cooperative binding, we demonstrate that the interaction between Gal4 and Tra1 occurs predominantly on the promoter and not off DNA. Finally, we show that although Tra1 is also targeted by other activators, these interaction are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction.

2011-12-30 | GSE31389 | GEO

GAL4-responsive genes in larval salivary glands of late Drosophila prepupae

Project description:The yeast transcription factor GAL4 has been reported to cause cell death and to have other biological effects when expressed in Drosophila (Kramer and Staveley, 2003: Genet. Mol. Res. 2, 43; Rezaval et al., 2007: Eur. J. Neurosci. 25, 683). Using heat-shock-induced expression of GAL4 to drive expression of a UAS-senseless responder gene in transcriptional profiling experiments, we found that the underlying cause of these effects might be a genomic response to GAL4. To further characterize this response and to account for GAL4-independent changes caused by the transgene integration, GAL4 was expressed from two copies of the transgene in two independent lines, P{GAL4-Hsp70.PB}89-2-1 (short P{hs-GAL4}89) and P{hs-GAL4}X1. In addition, GAL4 was expressed from only one copy of the transgene in P{hs-GAL4}89 prepupae to account for the dosage dependence of observed effects. Prepupae carrying the hs-GAL4 transgenes were subjected to a 30-min heat shock treatment (37 °C) at 9 hours after puparium formation. RNA was isolated from salivary glands dissected from these and similarly treated w1118 control animals at 14 hours after puparium formation and subjected to microarray analysis with Affymetrix GeneChips. The microarray data identified an overlapping set of 1,009 genes that showed an at least 1.5-fold change in expression in both of the GAL4-expressing lines, defining a core set of GAL4-responsive genes in the salivary glands. This set includes genes involved in the control and execution of programmed cell death and in other important regulatory pathways. Keywords: ectopic expression experiment

2008-05-29 | GSE8620 | GEO

Cell-type–resolved RNA-seq reveals molecular engrams of sexual experience in Drosophila neuromodulatory neurons

Project description:This study examined how different social/sexual experiences shape gene expression in defined neuromodulatory neurons of Drosophila melanogaster males. We profiled serotonergic (TRH), octopamine/tyramine (Tdc2), and neuropeptide F receptor (NPFR) neurons following three distinct conditions known to modulate motivational states: repeated successful mating, repeated sexual rejection, or social isolation (“naïve-single”). Cell-type-specific nuclei were isolated using the INTACT method, expressing an UNC-84-GFP nuclear tag under NPFR-Gal4, TRH-Gal4, or Tdc2-Gal4 drivers. RNA was extracted from 100 male brains per condition, with three biological replicates each, and subjected to bulk RNA-seq. Differential expression analysis revealed distinct transcriptional programs associated with mating outcomes and neuronal identity, identifying molecular pathways linked to chromatin regulation, metabolism, and synaptic remodeling. This dataset provides a resource for exploring how social experience is encoded in neuromodulatory circuits.

2025-12-03 | E-MTAB-15973 | biostudies-arrayexpress

Gene expression profiling of murine skin transgenically generated to express HPV16 E7 oncoprotein

Project description:A validated animal model would assist with research on the immunological consequences of the chronic expression of HPV16 E7 oncoprotein expression. Here we performed RNA-seq analysis of normal C57BL/6 skin versus mouse transgenically generated to express HPV16 E7 oncogene under the keratin 14 promoter (K14E7). Mice present with epithelial hyperplasia and increased immune cell infiltrate associated with immune suppression.

2017-10-30 | GSE102007 | GEO

Gene expression profile of Tra1 GID (Gal4 interaction defective) mutants

2011-12-30 | E-GEOD-31389 | biostudies-arrayexpress

Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4

Project description:Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4

| PRJNA145875 | ENA

RNA sequencing of nos-gal4>phf7ey Drosophila ovaries

Project description:Next generation sequencing of mRNA for nos-gal4>phf7ey Drosophila ovaries

2020-08-28 | GSE150213 | GEO

Genome-wide analysis of gene transcription in myocardium of mice transgenically expressing human cardiolipin synthase 1 (hCLS1) in cardiac tissue

Project description:Analysis of the transcriptome of cardiac tissue from mice transgenically expressing human cardiolipin synthesis. The hypothesis tested was that cardiac specific transgenic expression of cardiolipin synthase alters myocardial lipidomic flux resulting in compensatory metabolic gene transcriptional changes that will attenuate pathological environmental and dietary insults on bioenergetics.

2011-11-05 | GSE33451 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data