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Single-cell RNA-sequencing of wildtype (ME49∆KU80) or mutant (ME49∆KU80∆BFD1) parasites under unstressed or stressed conditions

ABSTRACT: Single-cell RNA-sequencing of wildtype (ME49∆KU80) or mutant (ME49∆KU80∆BFD1) parasites under unstressed or stressed conditions

PROVIDER: PRJNA546578 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR9204250_1.fastq.gz	Fastqsanger.gz
	SRR9204250_2.fastq.gz	Fastqsanger.gz
	SRR9204260_1.fastq.gz	Fastqsanger.gz
	SRR9204260_2.fastq.gz	Fastqsanger.gz
	SRR9204251_1.fastq.gz	Fastqsanger.gz

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Single-cell RNA-sequencing of wildtype (ME49∆KU80) or mutant (ME49∆KU80∆BFD1) parasites under unstressed or stressed conditions

Project description:To profile cell-cycle progression and the asynchronous process of differentiation, we performed single-cell RNA-sequencing (scRNA-Seq) of T. gondii using Seq-Well (Gierahn et al., 2017). Wild-type or ∆BFD1 parasites were grown under unstressed or stressed conditions (24, 48 or 72 h), mechanically released from host cells, and analyzed.

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