Project description:Duckweeds are small, rapidly growing aquatic flowering plants. Due to their ability for biomass production at high rates they represent promising candidates for biofuel feedstocks. Duckweeds are also excellent model organisms because they can be maintained in well-defined liquid media, usually reproduce asexually, and because genomic resources are becoming increasingly available. To establish a framework for quantitative metabolic research in duckweeds we derived a central carbon metabolism network model of Lemna gibba based on its draft genome. Lemna gibba fronds were grown in a photomixotrophic mode in liquid media under continuous light with 13C-labeled glucose as a carbon source. Two different conditions (nitrate vs. glutamine as nitrogen source) were compared by quantification of growth kinetics, metabolite levels, metabolic flux and transcript abundance.
Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..
Project description:Primary objectives: Characterization of the macrophage population subset that is modulated by enteric neurons
Primary endpoints: Characterization of the macrophage population subset that is modulated by enteric neurons via RNA sequencing
Project description:Intervention type:DRUG
Name of intervention:Huaier
Dose form / Japanese Medical Device Nomenclature:GRANULES
Route of administration / Site of application:ORAL
Dose per administration:20?
g
Dosing frequency / Frequency of use:OTHER, SPECIFY
20g? per day
Planned duration of intervention:3 months to extending if necessary
Intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary
detailes of teratment arms:hepatocellular carcinoma, breast cancer, colorectal cancer and related gastrointestinal cancers, urologic cancers including prostate cancer, pancreas cancer, and lung cancer, etc.
Comparative intervention name:None
Dose form / Japanese Medical Device Nomenclature:
Route of administration / Site of application:
Dose per administration:
Dosing frequency / Frequency of use:
Planned duration of intervention:
Intended dose regimen:
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.
Study Design: Comparative test, None, No, open(masking not used), EXPLORATORY
Project description:In flowering plants, silencing of transposable elements (TEs) is achieved by the installation of DNA methylation and histone modifications. 24-nt long small-interfering RNAs (siRNAs) guide the deposition of DNA methylation through RNA-directed DNA methylation (RdDM), which can be maintained independently of siRNAs in coordination with H3K9me2. In most angiosperms, RdDM is ubiquitously expressed in vegetative and sexual reproductive tissues. Spirodela polyrhiza (Lemnaceae), represents an exception with low levels of DNA methylation, very low expression of RdDM and near absence of 24-nt siRNAs during its clonal vegetative propagation. Moreover, some components of RdDM, DNA methylation maintenance and RNA silencing are absent from the genome. By investigating the distribution of epigenetic marks on TEs, we show that Spirodela epigenome is shaped by the loss of DNA methylation and H3K9me2 as TEs decay. Nonetheless, such abundant TE remnants remain silenced and marked by H3K9me1. In contrast, scarce, relatively intact TEs display high levels of DNA methylation, H3K9me2 and siRNAs whose patterns resemble those of TEs subjected to RdDM in other angiosperms. Furthermore, despite the absence of DCL2 in duckweeds, Spirodela can produce 22-nt siRNAs, not only from TEs, but from diverse sources of double-stranded (ds)RNA. Our data suggests that RdDM might still be functional during vegetative clonal growth, albeit tissue or developmentally regulated, and highlights the use of alternative models to further understand and explore the diversity of silencing pathways in plants.
Project description:Analysis of the covalent attachment of GMP to the RNA dependent RNA polymerase proteins of equine arteritis virus and SARS-CoV-2 proteins using heavy-isotope assisted MS and MS/MS peptide sequencing.