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Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites

ABSTRACT: Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites

PROVIDER: PRJNA548563 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9289100.fastq.gz	Fastqsanger.gz
	SRR9289101.fastq.gz	Fastqsanger.gz
	SRR9289102.fastq.gz	Fastqsanger.gz
	SRR9289103.fastq.gz	Fastqsanger.gz
	SRR9289104.fastq.gz	Fastqsanger.gz

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Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites

Project description:Kinetoplastids are a group of parasite species, several of which cause important diseases in human and livestock. Nearly all of these pathogenic species are transmitted by insect vectors, in which the parasites undergo a specific developmental program. One shared event undergone by multiple species is adherence to insect tissue. This adhesion occurs by means of a hemidesmosome-like structure that is thus far uncharacterized. We have used the monoxenous parasite Crithidia fasciculata, which exclusively infects mosquitoes, to study this process of parasite adhesion in the insect. We have transcriptionally profiled adherent and swimming forms of the parasite that have been generated in vitro, and compared these profiles to the adhesive form in the mosquito. Using a dual-RNAseq approach, we have also identified several genes that are differentially regulated in infected versus uninfected mosquitoes, including several immune genes. This indicates that the mosquito is responding to the presence of the parasites.

2019-06-26 | GSE132641 | GEO

Crithidia fasciculata swimmers vs adherent cells

Project description:Comparison of whole cell lystates of swimming (motile) and adherent (stationary) Crithidia fasciculata. Cf-C1 strain parasites grown in standard Brain-Heart Infusion medium supplemented with Hemin and cultured at 28 ºC.

2024-08-23 | PXD055056 | Pride

Dramatic changes in gene expression in different forms of Crithidia fasciculata reveal potential mechanisms for insect-specific adhesion in kinetoplastid parasites.

Project description:Kinetoplastids are a group of parasites that includes several medically-important species. These human-infective species are transmitted by insect vectors in which the parasites undergo specific developmental transformations. For each species, this includes a stage in which parasites adhere to insect tissue via a hemidesmosome-like structure. Although this structure has been described morphologically, it has never been molecularly characterized. We are using Crithidia fasciculata, an insect parasite that produces large numbers of adherent parasites inside its mosquito host, as a model kinetoplastid to investigate both the mechanism of adherence and the signals required for differentiation to an adherent form. An advantage of C. fasciculata is that adherent parasites can be generated both in vitro, allowing a direct comparison to cultured swimming forms, as well as in vivo within the mosquito. Using RNAseq, we identify genes associated with adherence in C. fasciculata. As almost all of these genes have orthologs in other kinetoplastid species, our findings may reveal shared mechanisms of adherence, allowing investigation of a crucial step in parasite development and disease transmission. In addition, dual-RNAseq allowed us to explore the interaction between the parasites and the mosquito. Although the infection is well-tolerated, anti-microbial peptides and other components of the mosquito innate immune system are upregulated. Our findings indicate that C. fasciculata is a powerful model system for probing kinetoplastid-insect interactions.

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Microarray study of human adrenal zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA)

Project description:Learn about the transcriptome profiling of zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA) in human adrenals

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Microarray study of human adrenal zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA)

Project description:Learn about the transcriptome profiling of zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA) in human adrenals 21 pairs of zona fasciculata (ZF) and zona glomerulosa (ZG), and 14 paired aldosterone-producing adenomas (APAs) from 14 Conn’s syndrome patients and 7 phaeochromocytoma patients were assayed on the Affymetrix Human Genome U133 Plus 2.0 Array. Laser capture microdissection was used to acquire samples of ZF, ZG and APA as previously described (Azizan EA, et al. J Clin Endocrinol Metab. 2012;97:E819-E829). For differentiation of ZG from ZF, sections were stained with cresyl violet using the LCM Staining Kit (AM1935, Ambion, USA). Data processing and analysis was performed using AffymetrixGeneChip Command Console Software and PartekGenomicSuite 6.5 (Partek Inc., St. Louis, MO). Gene expressions were portrayed as the summarized log-signal of the Robust Multichip Average (RMA) with quantilenormalisation and median polish for probe set summarisation. Validation by qPCR was performed on genes >10 fold up-regulated in zona glomerulosa (compared to zona fasciculata) and >10 fold up-regulated in aldosterone-producing adenomas (compared to zona glomerulosa).

2015-09-15 | E-GEOD-64957 | biostudies-arrayexpress

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