Project description:This study investigates the molecular pathogenesis of Ceratocystis manginecans, a fungal pathogen responsible for wilt and canker disease in Acacia mangium. Using a TMT-based quantitative proteomics approach, protein expression was profiled at early stages (1 h and 24 h post-inoculation) following infection with two fungal isolates, CAM and CEP. A total of 205 proteins were identified, with 105 differentially expressed across conditions. The results highlight key virulence-related processes such as DNA repair, oxidative stress response, and host-specific metabolic adaptation. This dataset reveals valuable protein-protein interaction networks that underpin pathogenicity and may inform strategies for disease control in forest plantations.
Project description:The draft nuclear genomes of Diplodia sapinea, Ceratocystis moniliformis s. str., and C. manginecans are presented. Diplodia sapinea is an important shoot-blight and canker pathogen of Pinus spp., C. moniliformis is a saprobe associated with wounds on a wide range of woody angiosperms and C. manginecans is a serious wilt pathogen of mango and Acacia mangium. The genome size of D. sapinea is estimated at 36.97 Mb and contains 13 020 predicted genes. Ceratocystis moniliformis includes 25.43 Mb and is predicted to encode at least 6 832 genes. This is smaller than that reported for the mango wilt pathogen C. manginecans which is 31.71 Mb and is predicted to encode at least 7 494 genes. The latter is thus more similar to C. fimbriata s.str., the type species of the genus. The genome sequences presented here provide an important resource to resolve issues pertaining to the taxonomy, biology and evolution of these fungi.