Project description:To study the impact of TGF signaling on the transcriptome of vascular smooth muscle cells, we cultured human umbilical artery smooth muscle cells as 3D aggregates in hanging drops for 48 hours. One group was treated with TGFβRI/II inhibitor LY2109761 (10 μM, additional 10 μM after 24 h), the other group with solvent (DMSO) as control. RNA was isolated from both groups after 48h
Project description:3D cultivation of cells lead to changes in morphology of the cells. This is likely to explain the higher radioresistance of cells growing in 3D compared to cells growing in 2D cell culture. Whole genome gene expression is performed to determine genes involved in changes of cell moroholgy and radioresistance. Keywords: comparison of 2D vs. 3D cell culture
Project description:3D cultivation of cells lead to changes in morphology of the cells. This is likely to explain the higher radioresistance of cells growing in 3D compared to cells growing in 2D cell culture. Whole genome gene expression is performed to determine genes involved in changes of cell moroholgy and radioresistance. Keywords: comparison of 2D vs. 3D cell culture RNA of cells was isolated four days after growing in the two different cell culture systems
Project description:Prospective, open labelled, multicenter trial to evaluate the feasibility of ex vivo culture 3D (chemogram obtaining) on biopsies in order to estimate the predictive value of this technique for treatment response in patients treated by two different chemotherapies (FOLFOX or FOLFIRI) for colorectal cancer.
Project description:The naive CD4 T cell compartment is heterogeneous. Ly-6C- and Ly-6C+ Naive CD4 T cells were compare by microarrays. Ly-6C- and Ly-6C+ Naive CD4 T cells were purified for RNA extraction and hybridization on Affymetrix microarrays.
Project description:To investigate the differential transcriptomics upon DMT1 in 2D vs 3D cell culture in breast cancer cells Gene expression analysis from MDA-MB-231 RNA-seq data of WT and DMT1 KO cells both in 2D and 3D cell culture conditions
Project description:Global shotgun proteomic analysis using data independent acquisition (DIA) and Spectronaut analysis on the input cell lysates before the enrichment step in proximity labeling experiments, specifically MCF10A cells stably expressing Bcl2-associated agonist of cell death (BAD) with three serine to alanine mutations (S75A, S99A, S118A) fused with miniTurbo (miniTurbo-BAD3SA) in 2D with biotin treatment (n=3) and miniTurbo-BAD3SA in 3D with biotin treatment (n=4). The file "L20240424-miniTurbo-BAD-WT-vs-3SA-DIA.sne" contains Spectronaut search result including miniTurbo-BAD WT 2D and 3D shotgun samples found under the identifier MSV000094824 (2D) and MSV000094825 (3D).
Project description:BioID proximity labeling experiment on bait protein Bcl2-associated agonist of cell death (BAD) with three serine to alanine mutations (S75A, S99A, S118A) in MCF10A cell culture stably expressing BAD-miniTurbo.
2D cell culture: 20220805; sample: BAD3SA-turbo-biotin; control: BAD3SA-turbo (n=3); other raw files in the experiment (BAD-turbo-biotin, BAD-biotin, turbo-biotin, BAD-turbo) are found under the identifier MSV000093357. The file "20230921-2D-miniTurbo-BAD3SA-low-abundance-imputation.pdResult" contains the Proteome Discoverer search result for the full 2D miniTurbo experiment.
3D cell culture: 20230710; sample: BAD3SA-biotin (n=4); other raw files (BAD-turbo-biotin, turbo-biotin) in the experiment are found under the identifier MSV000093358. The file "20230921-3D-miniTurbo-BAD3SA-low-abundance-sampling.pdResult" contains the Proteome Discoverer search result for the full 3D miniTurbo experiment.
