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Microarray Analyses of lncRNA and mRNA in TAMs from breast cancer patient before or after chemotherapy

ABSTRACT: Microarray Analyses of lncRNA and mRNA in TAMs from breast cancer patient before or after chemotherapy

PROVIDER: PRJNA555834 | ENA |

REPOSITORIES: ENA

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Microarray Analyses of lncRNA and mRNA in TAMs from breast cancer patient before or after chemotherapy

Project description:Chemotherapies have been shown to enhance anti-tumor immunity, but whether chemotherapies affect tumor-associated macrophages (TAMs) is still uclear. We found TAMs is different before and after chemotherapy by comparing RNA profile of TAMs before and after chemotherapy.

2021-02-16 | GSE134600 | GEO

Microarray Analyses of mRNA and lncRNA in TAMs isolated from breast tumor of PyMT mice with or without chemotherapy

Project description:Chemotherapies have been shown to enhance anti-tumor immunity, but whether chemotherapies affect tumor-associated macrophages (TAMs) is still uclear. We found TAMs is different with or without chemotherapy by comparing RNA profile of TAMs with or without chemotherapy.

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Microarray Analyses of mRNA and lncRNA in TAMs isolated from breast tumor of PyMT mice with or without chemotherapy

Project description:Microarray Analyses of mRNA and lncRNA in TAMs isolated from breast tumor of PyMT mice with or without chemotherapy

| PRJNA555835 | ENA

Microarray Analyses of mRNA in lncRNA IRENA overexpressed macrophages

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The mRNA and lncRNA profiles of the monocytes-derived macrophages (MDMs) and tumor associated macrophages (TAMs)

Project description:We compared the lncRNA expression profile of MDMs and TAMs. TAMs were polarized by treating human monocytes-derived macrophages (MDMs) with the conditioned media of MDA-MB-231 cells.

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The mRNA and lncRNA profiles of breast cancer cells with or without TAMs coculture

Project description:We compared the lncRNA expression profile of breast cancer cells with or without TAMs coculture in transwell system.

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Project description:We compared the lncRNA expression profile of extracellular vesicle from MDMs and TAMs. TAMs were polarized by treating human monocytes-derived macrophages (MDMs) with the conditioned media of MDA-MB-231 cells.

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Gene expression profiles of tumor-associated macrophages (TAMs) overexpressing miR-511-3p

Project description:Hematopoietic stem/progenitor cells (HS/PCs) were transduced with lentiviral vectors overexpressing OFP and either miR-511-3p or a control, mutated miRNA sequence (miR-511-3p-mut). The transduced HS/PCs were then transplanted in recipient C57BL/6 mice. Tumors (Lewis lung carcinomas, LLC) were injected s.c. 4 weeks after HS/PC transplant. Lentiviral vector-transduced (OFP+), tumor-associated macrophages (TAMs) were isolated 4 weeks after LLC injection by fluorescence-activated cell sorting. Gene expression profiles of TAMs overexpressing either miR-511-3p or miR-511-3p-mut were obtained from 3 independent biological samples/each. Gene expression profiles of miRNA-overexpressing TAMs were then compared with the gene expression profiles of wild-type TAMs isolated from LLCs grown in nontransplanted C57BL/6 mice; the latter TAMs were subfractioned into MRC1+CD11c(low) or CD11c+MRC1(low) subsets before RNA isolation and analysis. Comparison of gene expression profiles of TAMs revealed that miR-511-3p overexpression tunes down the expression of multiple genes that are classically upregulated in protumoral MRC1+CD11c(low) TAMs. TAMs were isolated from Lewis lung carcinomas grown s.c. in C57BL/6 mice.

2012-04-01 | E-GEOD-34903 | biostudies-arrayexpress

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