Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hippocampal gene expression profiling in mice treated with stigmasterol or beta-sitosterol

ABSTRACT: Hippocampal gene expression profiling in mice treated with stigmasterol or beta-sitosterol

PROVIDER: PRJNA555893 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9701630.fastq.gz	Fastqsanger.gz
	SRR9703360.fastq.gz	Fastqsanger.gz
	SRR9703370.fastq.gz	Fastqsanger.gz
	SRR9701631.fastq.gz	Fastqsanger.gz
	SRR9703361.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Hippocampal gene expression profiling in mice treated with stigmasterol

Project description:Anxiety and stress-related conditions represent a significant health burden in modern society. Unfortunately, most of the drugs currently used in the clinic modulate neurotransmission and are prone to side effects, limiting their long-term use. We used bioinformatics screening to find small molecules mimicking the transcriptional profile recently reported in hippocampi of importin α5 mutant anxiolytic mice, identifying the plant sterol β-sitosterol as a top candidate. Behavioral analyses confirmed anxiolytic properties of β-sitosterol, but did not show anxiolytic activity for stigmasterol, a related plant sterol. The present RNA-seq experiment characterizes the hippocampal transcriptome in mice 1 hr and 6 hrs after after intraperitoneal injection of 100 mg/kg stigmasterol or vehicle.

2021-05-18 | GSE134460 | GEO

Hippocampal gene expression profiling in mice treated with beta-sitosterol

Project description:Anxiety and stress-related conditions represent a significant health burden in modern society. Unfortunately, most of the drugs currently used in the clinic modulate neurotransmission and are prone to side effects, limiting their long-term use. We used bioinformatics screening to find small molecules mimicking the transcriptional profile recently reported in hippocampi of importin α5 mutant anxiolytic mice, identifying the plant sterol β-sitosterol as a top candidate. Behavioral analyses confirmed anxiolytic properties of β-sitosterol. The present RNA-seq experiment characterizes the hippocampal transcriptome in mice 1 hr and 6 hrs after intraperitoneal injection of 100 mg/kg β-sitosterol or vehicle.

2021-05-18 | GSE134467 | GEO

Hippocampal gene expression profiling in mice treated with beta-sitosterol

Project description:Hippocampal gene expression profiling in mice treated with beta-sitosterol

| PRJNA555199 | ENA

Hippocampal gene expression profiling in mice treated with stigmasterol

Project description:Hippocampal gene expression profiling in mice treated with stigmasterol

| PRJNA555411 | ENA

RNAseq sequencing signatures of Diffuse Large B-cell Lymphoma cells after beta-sitosterol treatment

Project description:Resistance to Rituximab is a significant challenge in treating Diffuse Large B-cell Lymphoma (DLBCL). β-Sitosterol (β-ST) is a plant sterol that has been found in a broad variety of fruits, spices, and medicinal plants. While the anti-neoplastic properties of β-Sitosterol (β-ST) are established in various solid malignancies, its impact on DLBCL is uncharted. To further investigation the mechanism of β-ST on DLBCL, we employed RNAseq transcriptome analysis to detect differential expression genes (DEGs) between β-ST treated and untreated DLBCL cells. Our findings indicate that β-ST impedes DLBCL cell proliferation in a concentration and time-dependent manner. It appears to alter sphingolipid metabolism, facilitate (ASM translocation to the plasma membrane, augment ceramide platforms through increased ceramide synthesis, and consequently precipitate apoptosis in DLBCL cells.

2024-12-01 | GSE252371 | GEO

Quantitative proteomic analysis of hippocampal tissues from mice treated with a CK1δ/ε inhibitor

Project description:Hippocampal tissues from 3xTg-AD mice treated with a CK1δ/ε inhibitor or vehicle, and non-transgenic mice treated with vehicle, were harvested and processed for proteomic analysis using label-free quantification.

2019-07-30 | PXD012281 | Pride

To determine the effects of non-cholesterol based sterol enriched diets on liver gene expression

Project description:Xenosterol accumulation in mice deficient in sterolin function leads to significant toxicity, with infertility, decreased body fat accumulation, macrothrombocytopenia, cardiac fibrosis and premature death. The predominant xenosterols are phytosterols derived from the diet which includes a mixture of sitosterol (typically 70%), campesterol (~20%) and stigmasterol (~5-10%), and ergosterol found in fungi. To delineate whether ergosterol or stigmasterol could accumulate in Abcg8 knockout mice and demonstrate any signs of xenosterol toxicity, we designed two diets with one supplemented with ergosterol (>98% purity) and other with stigmasterol (>80% purity) and fed them to Abcg8 knockout mice. Over a 12-week period, both male and female Abcg8 knockout mice fed with either of the diets gained normal amounts of weight, body fat, showed no disturbances in tail-cuff measured blood pressure, and plasma analyses showed no abnormalities of platelet counts or volumes, blood glucose, or plasma cholesterol. Fertility testing showed no abnormalities. While stigmasterol accumulation was observed in the both plasma and tissues, elevated levels of ergosterol was not observed in plasma. However, there was a dramatic increase in plasma brassicasterol, with levels reaching 80 mg/dL in plasma. Our data concludes that either stigmasterol or ergosterol or brassicasterol accumulations in Abcg8 Knockout Mice does not account for xenosterol toxicity.

2020-09-04 | GSE146522 | GEO

TGF-beta treated airway epithelium

Project description:TGF-beta treated airway epithelium

| PRJNA783342 | ENA

Transcriptome sequencing analysis of BRAF-mutant melanoma metastases.

Project description:Melanoma patients carry a high risk of developing brain metastases, and improvements in survival are still measured in weeks or months. Durable disease control within the brain is impeded by poor drug penetration across the blood-brain barrier, as well as intrinsic and acquired drug resistance. In this study, we used high-throughput pharmacogenomic profiling to identify potentially repurposable compounds against BRAF-mutant melanoma brain metastases. One of the compounds identified was β-sitosterol, a well-tolerated and brain-penetrable phytosterol. We found that β-sitosterol attenuated melanoma cell growth in vitro and significantly inhibited brain metastasis in vivo. Large-scale phosphoproteomic and in silico analyses indicated that the therapeutic potential of β-sitosterol was linked to mitochondrial interference. Mechanistically, β-sitosterol effectively reduced mitochondrial respiration and respiratory capacity in melanoma cells, mediated by a selective inhibition of mitochondrial complex I. This led to increased oxidative stress and apoptosis. Notably, we observed completely abrogated BRAF inhibitor resistance when vemurafenib was combined with either β-sitosterol or a functional knockdown of mitochondrial complex I. Based on its favorable tolerability, excellent brain bioavailability, and capacity to inhibit mitochondrial respiration, β-sitosterol represents a promising candidate for drug repurposing in patients with, or at risk for, melanoma brain metastases.

2019-04-06 | GSE100066 | GEO

Transcription profiling of mouse vehicle and sAPPalpha-treated hippocampal slice cultures

Project description:Gene expression changes induced by alpha-secretase cleaved amyloid precursor protein (sAPPalpha) in organotypic hippocampal slice cultures of male, postnatal day 15 mice (C57B6/SJL). Hippocampal slice cultures were treated with phosphate buffered saline (GSM26700, GSM26701, GSM26702) or 1 nM sAPPalpha (GSM26703, GSM26704, GSM26705) for 24 h. Each sample consists of total RNA isolated from 8-12 slices from 4 mice. Data were analyzed with MAS 5.0 and scaled to 2500. sAPPalpha induces the amyloid sequestration protein transthyretin, insulin-like growth factor 2, insulin-like growth factor binding protein 2, and other genes involved in protective pathways such as apoptosis inhibition, detoxification, and retinol transport. See Stein, TD, Anders, NJ, DeCarli, C, Chan, SL, Mattson, MP, and Johnson JA. Neutralization of transthyretin reverses the neuroprotective effects of secreted APP in APPSw mice resulting in tau phosphorylation and loss of hippocampal neurons: support for the amyloid hypothesis. J Neurosci. in press.

2007-10-10 | E-GEOD-1555 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data