Diplonemid endosymbionts
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ABSTRACT:
PROVIDER: PRJNA556273 | ENA |
REPOSITORIES: ENA
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Project description:Through transcriptome profiling using RNA-seq, we investigated the mechanisms behind bacterial endosymbiont (Burkholderia rhizoxinica) control over host (Rhizopus microsporus) reproductive biology. By analyzing differential expression across six different conditions, including fungal opposite mates growing independently with or without endosymbionts, as well as opposite mates growing together with endosymbionts (mating) or without endosymbionts (no mating), we were able to identify that endosymbionts control expression of a Ras signaling protein critical for sexual reproduction in many fungi (Ras2). As little is known regarding sexual reproduction in Mucoromycotina, we also used these data to investigate conservation of sex-related genes across all fungi, as well as predict potential genes involved in sensing of trisporic acid, the mating pheromone used by these fungi. 6 different conditions were analyzed, each consisting of two biological replicates. These included Rhizopus microsporus ATCC52813 (sex +) growing alone with endosymbionts, R. microsporus ATCC52814 (sex -) growing alone with endosymbionts, ATCC 52813 growing alone without endosymbionts, ATCC52814 growing alone without endosymbionts, ATCC52813 and ATCC52814 growing together with endosymbionts (successfully mating), and ATCC52813 and ATCC52814 growing together without endosymbionts (failure to mate). In each condition, fungi were cultivated on half-strength PDA and plugs of mycelium were placed at the edge of the plate. After 6 days, approximately 2.5 cm of tissue were harvested from the center of the plate. Each biological replicate consists of 5 plates which were pooled prior to RNA extraction to ensure sufficient tissue was collected.
2017-05-13 | E-GEOD-57644 | biostudies-arrayexpress
Project description:Through transcriptome profiling using RNA-seq, we investigated the mechanisms behind bacterial endosymbiont (Burkholderia rhizoxinica) control over host (Rhizopus microsporus) reproductive biology. By analyzing differential expression across six different conditions, including fungal opposite mates growing independently with or without endosymbionts, as well as opposite mates growing together with endosymbionts (mating) or without endosymbionts (no mating), we were able to identify that endosymbionts control expression of a Ras signaling protein critical for sexual reproduction in many fungi (Ras2). As little is known regarding sexual reproduction in Mucoromycotina, we also used these data to investigate conservation of sex-related genes across all fungi, as well as predict potential genes involved in sensing of trisporic acid, the mating pheromone used by these fungi.
2017-05-13 | GSE57644 | GEO
Project description:Rhizopus microsporus is one of the most common agents of mucormycosis, a severe and emerging infection caused by Mucorales fungi that poses a significant clinical challenge, particularly due to the growing population of immunocompromised individuals. An substantial proportion of clinical isolates harbor bacterial endosymbionts, which regulate key fungal functions, such asexual sporulation. The strict dependence on endosymbionts for spore formation has limited a comprehensive understanding of endosymbiosis in R. microsporus biology. Here, we demonstrate that sporulation in endosymbiont-cured strains of this fungus, which previously harbored Mycetohabitans bacteria, can be induced by light. Interestingly, both light and endosymbionts regulate sporulation through the same regulatory pathway, and a high proportion of the genes upregulated by these signals are known to be involved in asexual sporulation in other fungi, including Mucorales. Light-induced sporulation enabled comparative assays to assess the impact of symbiosis on fungal traits. In addition to previously known effects on fungal fitness, this study reveals that germination is independent of endosymbionts, although their presence accelerates the process. Furthermore, it shows that asexual spores lacking endosymbionts exhibit a reduced virulence in a mouse model of mucormycosis. The discovery of light-induced sporulation in endosymbiont-free strains of R. microsporus paves the way for future comparative studies using genetically identical backgrounds, advancing our understanding of fungal-bacterial symbiosis and its role in fungal biology and human pathogenesis.
2026-02-28 | GSE289616 | GEO
Project description:Bahamas Lucinid Endosymbionts Metagenomes
| PRJNA481370 | ENA
Project description:Sequences of Coral Endosymbionts ITS Region Metagenome
| PRJNA778185 | ENA
Project description:Animal-algal photosymbioses are a unique group of symbiotic relationships in which animals harbor photosynthetic algae within their cells and tissues. Both marine and freshwater sponges host algal endosymbionts. In previous work, we demonstrated that freshwater sponges can acquire these endosymbionts horizontally through algal infection and that potentially conserved evolutionary pathways may lead to the establishment of the endosymbioses including those involved in endocytosis, ion transport, vesicle-mediated transport, innate immunity, redox regulation, and metabolic processes. Here, we show that algal symbionts can be transferred vertically from algal-bearing overwintering gemmules to adult sponges, and that their proliferation is enhanced by light. Sponges grown under light conditions harbored higher algal loads than those in the dark; however, algae were still able to proliferate and persist in sponges reared in the dark, occupying similar spatial locations to those grown in light. RNA-Seq analysis of algal-bearing sponges across developmental stages in light and dark conditions revealed genetic regulatory pathways involved in the transmission and establishment of the endosymbiosis. Differential expression analysis indicated that the endocytosis and SNARE pathways regulate the internalization and transport of algae at the earliest stage of hatching under light conditions and later in development under dark conditions, potentially contributing to the recruitment of endosymbiotic algae. In sponges hatched in the dark, genes involved in vesicle acidification are regulated, alongside changes in the expression of genes in the pentose phosphate pathway - a key metabolic route involved in redox homeostasis and circadian rhythm regulation via NADPH metabolism, is observed. E. muelleri serves as a versatile model system, supported by robust genomic and transcriptomic resources, for studying host-symbiont interactions. It offers a unique opportunity to investigate the molecular signaling and environmental factors that shape symbiosis in a system where the host can exist with or without algal endosymbionts, symbionts can be acquired either horizontally or vertically, and proliferation of the algae can occur with or without photosynthesis.
2025-11-19 | GSE302403 | GEO
Project description:Taxonomic assignment of algal endosymbionts from Amphistegina lobifera from the Red Sea
| PRJEB33832 | ENA
Project description:Wolbachia and Spiroplasma endosymbionts of Anurida maritima (Collembola)
| PRJNA860916 | ENA
Project description:Biosynthesis of mitochondrial genome-encoded proteins is carried out by the mitoribosome, a specialized apparatus that has evolved and diverged dramatically since its bacterial origin. Recent studies across various eukaryotes have demonstrated widespread structural and compositional diversity of mitoribosomes. We used sucrose gradient centrifugation and Blue-Native PAGE to separate mitoribosomes of Diplonema papillatum, the type species of diplonemids, a widespread group of single-celled marine flagellates.
2023-05-19 | PXD039882 | Pride
Project description:Taxonomic assignment of algal endosymbionts from Amphistegina gibbosa and Amphistegina lessonii exposed to heat stress.
| PRJEB24424 | ENA