Project description:Light exposure quickly inhibits the elongation of mesocotyl of rice seedling. The two corresponding periods from darkness to loght (D_5d, L_1h) were selected to perform degradome sequencing.
Project description:Light exposure quickly inhibits the elongation of mesocotyl of rice seedling. The two corresponding periods from darkness to loght (D_5d, L_1h) were selected to perform small RNA sequencing.
Project description:The mesocotyl, a structure separating the grass seedling cotyledon from the coleoptile, exerts a major influence over the seedling’s ability to emerge. Its elongation is inhibited by light. A transcriptomic comparison between mesocotyl tissue which had developed in the dark and which had been exposed to light identified a large number of differentially transcribed genes (DTGs)
Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. Tthe phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24.
Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.
Project description:rs09-09_della-dark - della-regulation in darkness versus light - Identification of DELLA-dependent downstream targets in darkness - Aim was to determine downstream target of DELLA proteins involved in skotomorphogenesis. Wt, ga1-3, ga1-3_rga_gai_rgl1_rgl2_rgl3 global seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4°C. Plates were placed in growth cabinet at 22°C for 5 days in darkness or in continous light. Keywords: gene knock out
Project description:Cyanophage infection outcomes depend strongly on host physiological state, yet the transcriptional dynamics of the P-SSP7–Prochlorococcus MED4 interaction under different light regimes remain poorly understood. This dataset captures the time-resolved transcriptional response of Prochlorococcus MED4 following infection with cyanophage P-SSP7 under three light conditions: standard light, high light, and darkness.