Project description:Transcriptome analysis was performed to determine what gene expression changes occur in response to treatment of the plant-derived compound harmine and to determine its effect on protein kinase C agonist reactivation of latent HIV.
Project description:After stimulation of RAW264.7 cells with LPS and Harmine, high-throughput sequencing technology was used to explore the changes in the inflammation level of RAW cells and the enrichment of signaling pathways after LPS and Harmine treatment, and to find an interesting target pathway.
Project description:RNA-seq was applied for identifying the change of transcriptome between U2OS cells with starvation-induced quiescence treated with Harmine Hydrochloride or INDY and the untreated controls.
Project description:Chemical inhibition of DYRK1A (e.g. using harmine) inhibits Th17 and promotes Treg differentiation. To better understand the mechanisms by which DYRK1A regulates Th17 differentiation, we are comparing expression profiles of naïve CD4+ T cells during the course of Th17 differentiation in the presence or absence of harmine.
Project description:K562 cells were treated with 20nM PMA. Cells were harvested after 48 hours and RNA was extracted with miRvana RNA isolation kit. Expression profiling was done on Agilent platform.
Project description:β-carboline alkaloids produced by Peganum harmala (Syrian rue) have been reported to possess antimicrobial, neurological, and anticancer activities, yet their biological role and molecular target remain unknown. We investigated the effects of the harmala alkaloid, harmaline, on plant growth and cell division using Arabidopsis thaliana as a model system. RNAseq was performed on Arabidopsis seedlings in control, harmaline, and harmine treatments.