Project description:Analysis of whole tumor mRNA with nanostring immune exhaustion platforrm Analyzed the expression of 785 genes from the total tumor RNA extracted from 16 solid murine tumors
Project description:Pathology of the most lethal form of malaria is caused by Plasmodium falciparum asexual blood stages and initiated by merozoite invasion of erythrocytes. We present a phosphoproteome analysis of extracellular merozoites revealing 1765 unique phosphorylation sites including 785 sites not previously detected in schizonts
Project description:To underly the potential downstream transcriptional regulation of HPIP that could account for cartilage and skeletal development. RNA-seq analysis were performed in HPIP knockout and control primary chondrocytes.Among the 1271 significantly differentially expressed genes, transcripts for 486 (7%) of them were upregulated while transcripts for 785 (11%) were downregulated in HPIP knockout chondrocytes compared to the controls. We found HPIP was closely correlated with the cartilage development.
Project description:After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hundreds of previously unknown heteromeric complexes. Lab Heads: Andrew Emili; andrew.emili@utoronto.ca ;Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada Mohan Babu; mohan.babu@uregina.ca ;Research and Innovation Centre, University of Regina, SK S4S OA2, Canada
Project description:We performed differential gene expression analysis using high throughput multiplex analysis via NanoString nCounter Immune Profiling panel, which analyzes 785 genes (including 12 internal reference genes) related to immune pathways. nSolver software was used to analyze the data and a heat map was generated to show differential expression of 773 genes. Further, using a fold cut-off of ≥1.5 and p-value <0.05, we observed that MZB1 KO significantly modulated the expression of multiple cancer immune genes.
Project description:The Arabidopsis thaliana REPRESSOR OF GA gene (RGA) encodes a DELLA protein that associates with multiple transcription factors to control plant growth in response to the hormone gibberellin (GA) (1). As part of a screen for genes that mediate the function of RGA in stem growth and shoot meristem function, we performed ChIP-seq to identify genome-wide loci associated with RGA in inflorescence apices. To detect genes controlled by RGA in a gain-of-function semi-dwarf background, we used a GFP-tagged, gibberellin-insensitive version of RGA (RGAp:GFP-rga-delta17) (2). (1) J.-M. Daviere, P. Achard, Gibberellin signaling in plants. Development 140, 1147-1151 (2013). (2) A. Dill, T. P. Sun, Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics 159, 777-785 (2001).
