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Identification of BBX proteins as rate-limiting co-factors of HY5

ABSTRACT: Identification of BBX proteins as rate-limiting co-factors of HY5

PROVIDER: PRJNA564640 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10089752.fastq.gz	Fastqsanger.gz
	SRR10089753.fastq.gz	Fastqsanger.gz
	SRR10089754.fastq.gz	Fastqsanger.gz
	SRR10089755.fastq.gz	Fastqsanger.gz
	SRR10089756.fastq.gz	Fastqsanger.gz

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Identification of BBX proteins as rate-limiting co-factors of HY5

Project description:As a source of both energy and environmental information, the importance for plants to monitor the incoming light is evidenced by the highly integrated nature of the light signal transduction pathway with numerous other pathways throughout plant development. One of these signal integrators is the bZIP transcription factor HY5 which holds a key role as a positive regulator of light signalling in plants. Although HY5 is described to act as a DNA-binding transcriptional regulator, the lack of any apparent transactivation domain makes it unclear how HY5 is able to accomplish its many functions. We describe the identification of three B-box containing proteins (BBX20, 21 and 22) as essential partners for HY5 dependent modulation of hypocotyl elongation, anthocyanin accumulation and transcriptional regulation. The bbx202122 triple mutant mimics the phenotypes of hy5 in the light and its strong suppression of the cop1 phenotype in darkness. Furthermore, RNA-seq experiments show that 84% of genes differentially regulated in bbx202122 are also HY5 regulated, consistent with the B-box proteins and HY5 acting interdependently.

2020-07-01 | GSE137147 | GEO

Mitochondrial reorganization is rate-limiting for direct cardiac reprogramming

Project description:Mitochondrial reorganization is rate-limiting for direct cardiac reprogramming

| PRJNA1120770 | ENA

Karrikins control seedling photomorphogenesis through the HY5-BBX transcriptional module.

Project description:Karrikins control seedling photomorphogenesis through the HY5-BBX transcriptional module.

| PRJNA702130 | ENA

Tcf21 functions as a rate-limiting factor during visceral adipose tissue development

Project description:Tcf21 functions as a rate-limiting factor during visceral adipose tissue development

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ELONGATED HYPOCOTYL (HY5) AND HY5 HOMOLOG (HYH) maintain shade avoidance suppression in sunlight

Project description:We show that longer-term inhibition of shade avoidance in Arabidopsis is sustained by ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) which, together, regulate transcriptional reprogramming of genes involved in hormone signalling and cell wall modification.

2023-06-07 | GSE192469 | GEO

Karrikins control seedling photomorphogenesis through the HY5-BBX transcriptional module.

Project description:Karrikins (KARs) are a class of butenolide molecules found in the smoke of burned plant material that can induce germination of many plant species that emerge after fire. Intriguingly, KAR perception is widely conserved and not limited to fire-followers. For example, germination of dormant Arabidopsis thaliana seeds can also be stimulated by KARs. Additionally, KAR treatment enhances responses of seedlings to light. These responses include inhibition of hypocotyl elongation, enhancement of cotyledon expansion, and transcriptional upregulation of light-responsive genes not only in Arabidopsis but also in Brassica tournefortii. Although the core KAR signaling mechanism, consisting of KAI2-SCF/MAX2-mediated degradation of SMAX1 and SMXL2, is well described, it remains elusive how SMXL degradation leads to downstream growth responses. In this study, we analyse the role of BBX20 in the KAR signaling pathway through both chemical and genetic approaches using knock-out mutants. We find that BBX20, together with its close homologue BBX21, is essential for KAR-induced inhibition of hypocotyl elongation and anthocyanin accumulation. Our detailed genetic analysis suggests that BBX20 and BBX21 act in a HY5-dependent transcriptional module downstream of SMAX1 and SMXL2. While RNA-seq analysis revealed large-scale transcriptional changes in the smax1 smxl2 mutant, our results clearly suggest that BBX20 and BBX21 are required for a subset of SMAX1/SMXL2-dependent transcriptional regulation. Overall, our data suggest that the KAR signaling pathway promotes the activity of the HY5-BBX transcriptional module and that this module represents a point of convergence between KAR and light signaling.

2021-09-06 | GSE166857 | GEO

Microarray analysis in wild type and hy5 plants

Project description:Gene expression profiles between wild type (WT) and hy5 were compared to know HY5-targets.

2015-01-06 | GSE62119 | GEO

Analysis of transcription factor HY5 genomic binding sites in Arabidopsis

Project description:The transcription factor HY5 acts downstream of multiple families of the photoreceptors and promotes photomorphogenesis. Although it is well accepted that HY5 acts to regulate target gene expression, in vivo binding of HY5 to any of its target gene promoters has yet to be demonstrated. Here we used a chromatin immunoprecipitation procedure to verify suspected in vivo HY5 binding sites. We demonstrated that in vivo association of HY5 with promoter targets is not altered under distinct light qualities or during light-to-dark transition. Coupled with DNA chip hybridization using high density 60-nucleotide oligomer microarray that contains one probe for every 500 nucleotides over the entire Arabidopsis genome, we mapped genome wide in vivo HY5 binding sites. This analysis showed that HY5 binds preferentially to promoter regions in vivo and revealed over 3 thousand chromosomal sites as putative HY5 binding targets. HY5 binding targets tend to be enriched in the early light responsive genes and transcription factor genes. Our data thus supports a model in which HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis. Keywords: ChIP-chip

2007-03-01 | GSE6510 | GEO

Title: Lysine acetylation modulates HY5 function in Arabidopsis under high light conditions

Project description:To determine the role of HY5 under HL in transcriptional regulation, RNA-seq was performed on 7-day-old Col-0 and hy5 mutant seedlings grown under GL and HL conditions. HY5-dependent and HL-responsive differentially expressed genes (DEGs) were identified by comparing Col-0 vs. hy5 under HL, and GL vs. HL conditions, respectively (Fig. S8A). Using a 2-fold change and p ≤ 0.05 cutoff, 6,929 and 7,378 DEGs were detected in Col-0 and hy5, respectively, with 4,130 genes overlapping (Fig. S8B). Volcano plot analysis identified 615 upregulated and 291 downregulated HY5-dependent genes under HL. Next, transcriptome analysis was conducted using RNA-seq on 7-day-old seedlings of Col-0, hy5, 35S:HY5K87K-GFP/hy5#1, 35S:HY5K87Q-GFP/hy5#1, and 35S:HY5K87R-GFP/hy5#2 grown under GL conditions. Comparison of DEGs between transgenic lines and the hy5 mutant revealed 3,755, 3,450, 5,341, and 5,935 upregulated, and 4,139, 3,584, 5,832, and 6,209 downregulated genes in Col-0 (native HY5), 35S:HY5K87K-GFP/hy5#1 (HY5K87K), 35S:HY5K87Q-GFP/hy5#1 (HY5K87Q), and 35S:HY5K87R-GFP/hy5#2 (HY5K87R) seedlings, respectively.

2026-01-05 | GSE308603 | GEO

Strigolactones positively regulate HY5-dependent autophagy and the degradation of ubiquitinated proteins in response to cold stress in tomato

Project description:Autophagy, involved in protein degradation and amino acid recycling, plays a key role in plant development and stress responses. However, the relationship between autophagy and phytohormones is unclear. We used diverse methods, including CRISPR/Cas9, UPLC-MS/MS, chromatin immunoprecipitation, electrophoretic mobility shift assays, and dual-luciferase assays to explore the molecular mechanism of strigolactones in regulating autophagy and the degradation of ubiquitinated proteins under cold stress in tomato. We show that cold stress induced the accumulation of ubiquitinated proteins. Mutants deficient in strigolactone biosynthesis were more sensitive to cold stress with a higher accumulation of ubiquitinated proteins, while treatment with the synthetic strigolactone analog GR245DS enhanced cold tolerance in tomato, with a higher accumulation of autophagosomes and transcripts of autophagy-related genes (ATGs), and a lower accumulation of ubiquitinated proteins. Meanwhile, cold stress induced ELONGATED HYPOCOTYL 5 (HY5) accumulation, which was triggered by strigolactones. HY5 further trans-activated ATG18a promoter, resulting in autophagy formation. Mutation of ATG18a compromised strigolactone-induced cold tolerance, followed by a decreased formation of autophagosomes and an increased accumulation of ubiquitinated proteins. These findings reveal that strigolactones positively regulate autophagy in an HY5-dependent manner and promote the degradation of ubiquitinated proteins under cold conditions in tomato.

2024-08-01 | PXD052107 | Pride

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