Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide expression analysis in murine liver after Nbs1 induced knockout

ABSTRACT: Genome-wide expression analysis in murine liver after Nbs1 induced knockout

PROVIDER: PRJNA565740 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10124938.fastq.gz	Fastqsanger.gz
	SRR10124939.fastq.gz	Fastqsanger.gz
	SRR10124940.fastq.gz	Fastqsanger.gz
	SRR10124941.fastq.gz	Fastqsanger.gz
	SRR10124942.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Genome-wide expression analysis in murine liver after Nbs1 induced knockout

Project description:NBS1 (Nbn in Mus musculus) is a critical component of the MRN (MRE11/RAD50/NBS1) complex, which regulates ATM- and ATR-mediated DNA damage response (DDR) pathways. NBS1 mutations cause the human genomic instability syndrome Nijmegen Breakage Syndrome (NBS), in which microcephaly and intellectual disability are marked neuronal deficits. NBS1 is essential for life, because of its function in the DDR to ensure proliferation and preventing the cell death of replicating cells. However, the function of NBS1 in postmitotic cells is unclear. To explore the possible role of Nbs1 in non-dividing cells and the effection of its deletion on gene expression, RNA-seq was carried out with Nbs1 induced knockout liver samples, in which most cells are postmitotic.

2022-09-16 | GSE137505 | GEO

DNMT1 KNOCKOUT IN MURINE LIVER

Project description:DNMT1 KNOCKOUT IN MURINE LIVER

| PRJNA280860 | ENA

RAD51 knockout in murine liver (4-week)

Project description:RAD51 knockout in murine liver (4-week)

| PRJNA1019234 | ENA

RAD51 knockout in murine liver (11-week)

Project description:RAD51 knockout in murine liver (11-week)

| PRJNA1019697 | ENA

NBS1 facilitates pre-ribosomal RNA biogenesis

Project description:Mutations of NBS1 gene result in Nijmegen breakage syndrome (NBS), and the gene encodes NBS1 that forms a complex with MRE11 and RAD50 and participates in DNA damage repair. However, the molecular mechanism by which the mutations of NBS1 cause clinical phenotypes of NBS, such as craniofacial dysmorphism, is still unclear. Here, we show that NBS1 localizes at the rDNA loci in the nucleoli and interacts with ribosome RNA (rRNA) transcription machinery including RNA polymerase I (Pol I) and TCOF1. Loss of NBS1 impairs Pol I-dependent transcription of pre-rRNA and induces nucleolar stress. In particular, lacking Nbs1 in mouse neural crest cells not only leads to the reduction of ribosome biogenesis but also craniofacial abnormalities during prenatal development. Moreover, the C-terminus of NBS1 is associated with pre-rRNA and a number of pre-rRNA processing factors, which may also facilitate pre-rRNA maturation. Taken together, our study reveals the functions of NBS1 in rRNA biogenesis.

2025-09-15 | PXD056042 | Pride

Murine Western diet-induced nonalcoholic steatohepatitis liver proteome

Project description:Non-alcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease worldwide, with 25% of these patients developing nonalcoholic steatohepatitis (NASH). NASH significantly increases the risk of cirrhosis and decompensated liver failure. Past studies in rodent models have shown the knockout of glycine-N-methyltransferase (GNMT) results in rapid pro-gression of steatosis, fibrosis, and hepatocellular carcinoma. However, the attenuation of GNMT in subjects with NASH and the molecular basis for its impact on the disease process are still unclear. To address this knowledge gap, we show the reduction of GNMT protein levels in the liver of NASH subjects compared to healthy controls. To gain insight into the impact of decreased GNMT in the disease process, we performed global label-free proteome studies on the livers from a murine Western diet-based model of NASH. Histological and molecular characterization of the animal model demonstrate high resemblance to the human disease.

2022-04-04 | PXD030781 | Pride

RNA-Seq analysis of KLF3 knockout E14.5 TER119+ (erythroid) murine fetal liver cells

Project description:The aim of this study was to analyze the transcriptome of TER119+ fetal liver cells in the absence of the transcription factor KLF3 at murine embryonic day E14.5 Three wildtype (WT; Klf3+/+) and three knockout (KO; Klf3-/-) samples

2014-02-23 | E-GEOD-50554 | biostudies-arrayexpress

Gene expression data from murine liver samples comparing hormone sensitive lipase (HSL) knockout mice vs. wildtype mice

Project description:HSL is a key enzyme in in the mobilization of fatty acids from the triglyceride stores of white adipose tissue. In addition, it is expressed in mice liver. In the present microarray study, changes in the transcript profile of murine liver samples due to global HSL knockout were investigated. Keywords: genetic modification

2009-01-03 | GSE10067 | GEO

Genome-wide expression analysis in murine brain in E14.5 vs. 10.5m old mice

Project description:NBS1 (Nbn in Mus musculus) is a critical component of the MRN (MRE11/RAD50/NBS1) complex, which regulates ATM- and ATR-mediated DNA damage response (DDR) pathways. NBS1 mutations cause the human genomic instability syndrome Nijmegen Breakage Syndrome (NBS), in which microcephaly and intellectual disability are marked neuronal deficits. NBS1 is essential for life, because of its function in the DDR to ensure proliferation and preventing the cell death of replicating cells. However, the function of NBS1 in postmitotic cells is unclear. To explore the possible role of Nbs1 in non-dividing cells and the effection of its deletion on gene expression, RNA-seq was carried out to compare the expression of Nbs1 and other DDR molecules in developing and adult brain.

2020-08-20 | GSE137506 | GEO

Quantitative Proteomics of human relevance of the dietary fiber-induced liver cancer murine model

Project description:Quantitative Proteomics of human relevance of the dietary fiber-induced liver cancer murine model

2020-06-06 | MSV000085559 | MassIVE