Ontology highlight
ABSTRACT:
PROVIDER: PRJNA577484 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR10276400.fastq.gz | Fastqsanger.gz | |||
| SRR10276401.fastq.gz | Fastqsanger.gz | |||
| SRR10276402.fastq.gz | Fastqsanger.gz | |||
| SRR10276403.fastq.gz | Fastqsanger.gz | |||
| SRR10276404.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 17 |
Similar Datasets
Project description:Diploptera punctata gut microbiome_2019 Raw sequence reads
| PRJNA598044 | ENA
Project description:Diploptera punctata isolate:Stay&Tobe Genome sequencing and assembly
| PRJNA803029 | ENA
Project description:RNAseq analysis of CD8 T cells after pregnancy-induced tolerance/hypofunction. The goal of this experiment was to compare the transcriptional effect of pregnancy on naive vs. memory fetus-specific CD8 T cells. We also included naive and skin-sensitized CD8 T cells (without pregnancy) as controls.
2023-05-03 | GSE216301 | GEO
Project description:In this study, we characterize the proteomic landscape of the invasive, edible, and bloom-forming jellyfish Phyllorhiza punctata by analyzing its oral arms, gonads, and mantle tissues. Utilizing high-throughput liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), we present a comprehensive profile of the protein composition across these tissues. Our findings provide novel insights into the functional and ecological roles of the P. punctata proteome, advancing the understanding of its biology and potential biotechnological applications.
2025-08-11 | PXD066818 | Pride
Project description:We aimed with this study through RNAseq, found diferentially expressend genes (DEGs) that can be used as a new pregnancy markers in peripheral blood mononuclear cells (PBMC) on day 18 post artificial insemination; and to assess the mRNA profile by RT-qPCR of new pregnancy markers in PBMC and peripheral blood polymorphonuclear cells (PMN) at early pregnancy Methods: Nelore heifers (N=21) were artificially inseminated in a fixed time (D0). On Day 10, 14, 16, 18 and 20, blood was collected for isolation of PBMC and PMN, and P4 concentration assay and color Doppler ultrasonography was performed to evaluate the corpus luteum (CL) . Pregnancy diagnosis was done on Day 28 and heifers were classified in pregant (N=9) and non-pregant (N=12). Heifers (N=6/group) with different (P<0.05) plasma P4 concentration, CL area and blood perfusion on Day 18 were selected and RNAseq was done on PBMC samples. RNAseq analysis indicated 220 DEGs (200 up regulated in P). Twenty DEGs found on RNAseq of PBMC with the highest fold-changes or no overlapping between pregant and non-pregant, were assessed by RT-qPCR from Day10 to 20 (N=6/group). Conclusions: nine DEGs studied by RT-qPCR presented increased expression on PBMC for Pregnant group in at least one-time point from Day16 to 20; but only 4 of these DEGs retained the expression increased on PMN (IFI6, IFI44, RSAD2 and LGALS3BP). Thus, results indicate potential genes to be used as novel pregnancy predictors in immune cells in cattle at early gestation.
2019-08-22 | GSE136102 | GEO
Project description:The aims of this study were to 1) identify the earliest transcriptional response of the bovine endometrium to the presence of the conceptus (using RNAseq), 2) investigate if these genes are regulated by interferon tau (IFNT) in vivo, and 3) determine if they are predictive of the pregnancy status of postpartum dairy cows. RNAseq identified 459 differentially expressed genes (DEGs) between pregnant and cyclic endometria on day 16. Quantitative real-time PCR analysis of selected genes revealed PARP12, ZNFX1, HERC6, IFI16, RNF213, and DDX58 expression increased in pregnant compared with cyclic endo- metria on day 16 and were directly upregulated by intrauterine infusion of IFNT in vivo for 2 h (P < 0.05). On day 13 following estrous endometrial expression of nine genes increased [ARHGAP1, MGC127874, LIMS2, TBC1D1, FBXL7, C25H16orf71, LOC507810, ZSWIM4, and one novel gene (ENSBTAT00000050193)] and seven genes decreased (SERBP1, SRGAP2, AL7A1, TBK1, F2RL2, MGC128929, and WBSCR17; P < 0.05) in pregnant compared with cyclic heifers. Of these DEGs, significant differences in expression between pregnant and cyclic endometria were maintained on day 16 for F2RL2, LIMS2, LOC507810, MGC127874, TBC1D1, WBSCR17, and ZSWIM4 (P < 0.05) both their expression was not directly regulated by IFNT in vivo. Analysis of the expression of selected interferon-stimulated genes in blood samples from postpartum dairy cows revealed a significant increase (P < 0.05) in expression of ZXFX1, PARP12, SAMD9, and HERC6 on day 18 following artificial insemination in cows subsequently confirmed pregnant compared with cyclic controls. In conclusion, RNAseq identified a number of novel pregnancy-associated genes in the endometrium of cattle during early pregnancy that are not regulated by IFNT in vivo. In addition, a number of genes that are directly regulated by short term exposure to IFNT in vivo are differentially expressed on day 18 following estrus detection in the blood of postpartum dairy cows depending on their pregnancy status. mRNA profiles of pregnant vs not pregnant cross-bred beef heifers at days 13 and 16 (n=5 per group)
2014-04-01 | E-GEOD-56392 | biostudies-arrayexpress